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  • 1
    Digitale Medien
    Digitale Medien
    Identification of IL-1β-induced messenger RNAs in rat pancreatic beta cells by differential display of messenger RNA (1999)
    Springer
    Diabetologia 42 (1999), S. 1199-1203 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Keywords Beta cells ; chemokine ; phospholipase-D ; DDRT-PCR ; interleukin-1 ; monocyte chemoattractant protein-1 ; adenine nucleotide translocator ; CINC-1 ; CINC-3.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Aims/hypothesis. Interleukin-1β is a putative mediator of pancreatic beta-cell dysfunction and damage in Type I (insulin-dependent) diabetes mellitus. To better understand the molecular mechanisms involved in IL-1β effects, we carried out a differential display of mRNA by RT-PCR to identify novel cytokine-regulated genes. Methods. Fluorescence activated cell sorting-purified rat pancreatic beta-cells were exposed for 6 or 24 h to IL-1β. Differentially expressed cDNA bands were cloned and then identified by comparing their sequences with data from the GenBank. Differential gene expression was confirmed by RT-PCR using specific primers. Results. Interleukin-1β increased the expression of adenine nucleotide translocator-1, phospholipase D-1 and cytokine-induced neutrophil chemoattractant-1 and decreased expression of the protein tyrosine phosphatase-like protein IA-2. Interleukin-1β-induced differential expression of these genes in beta cells was confirmed by RT-PCR. In additional studies, IL-1β was shown to induce chemokines other than cytokine-induced neutrophil chemoattractant-1, including cytokine-induced neutrophil chemoattractant-3 and monocyte chemotactic protein-1. Conclusion/interpretation. Our observations indicate that IL-1β modifies the expression of several genes in pancreatic beta cells. These genes may affect both function, viability and beta-cell recognition by the immune system. Functional characterization of the mRNAs which have been identified could facilitate a better understanding of the mechanisms leading to beta-cell destruction in Type I diabetes. [Diabetologia (1999) 42: 1199–1203]
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001250051292
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor (1999)
    Springer
    Cytotechnology 29 (1999), S. 215-220 
    Details
    ISSN: 1573-0778
    Schlagwort(e): CHO ; cytoline microcarrier ; fluidized bed bioreactor (FBR) ; stirred tank bioreactor (STR)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A recombinant Chinese hamster ovary clone was cultivated in a 2L Cytopilot Mini fluidized bed bioreactor using Cytoline 1 microcarriers and a 10L B. Braun stirred tank bioreactor with Cytodex 1 microcarriers. Cytoline 1 is a macroporous polyethylene microcarrier and Cytodex 1 is a solid DEAE-dextran microcarrier. Cytoline 1 microcarriers in the fluidized bed bioreactor were gently mixed by an uplifting flow. Circulation and sparging in Cytopilot Mini were separated from the fluidized microcarrier bed. Cytopilot Mini bioreactor with Cytoline 1 microcarriers offered 2.3 times more surface area than the stirred tank bioreactor. The 2L fluidized bed bioreactor accommodated approximately half the cells in the 10L stirred tank bioreactor. Moreover, Cytopilot Mini had approximately three times more product output rate and 5.5 times higher specific productivity than the stirred tank bioreactor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008064217040
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  • 3
    Digitale Medien
    Digitale Medien
    Concentration and distribution of copper and cadmium in water, sediments, detritus, plants and animals in a hardwater lowland river (1996)
    Springer
    Hydrobiologia 341 (1996), S. 71-80 
    Details
    ISSN: 1573-5117
    Schlagwort(e): Copper ; Cadmium ; distribution ; freshwater
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The concentration and distribution of copper (Cu) and cadmium (Cd) were examined in water, sediments, detritus, plants and animals in a small, lowland, hardwater river. Consistently higher concentrations of Cu and Cd were found in all types of samples from two sites. There were marked variations in metal concentrations between different types of samples, and between seasons. Copper and Cd were mainly concentrated in sediments, organic detritus and biota, while concentrations in water were three orders of magnitude lower than in the other components in the system. The relatively high concentrations of Cu and Cd in biota suggests that they provide an important pathway for metal transport through the food web in this particular hardwater river. From the rank order of concentrations it appears that sticklebacks exert a greater degree of control than invertebrates in the uptake and elimination of Cu and Cd.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00012305
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