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  • 1995-1999  (2)
  • Caffeine  (1)
  • Embryo sac  (1)
  • Freeze substitution  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 12 (1999), S. 67-70 
    ISSN: 1432-2145
    Keywords: Key words Cytoskeleton ; Cytoskeleton proteins ; Cytoskeleton function ; Pollen tube ; Embryo sac
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sexual reproduction in plants is intimately connected to the activity of the cytoskeletal apparatus in reproductive cells. Because of the ease with which the pollen tube can be studied, it has become a model for studying many aspects of cell physiology related to the cytoskeleton, such as movement of organelles and vesicles and cell division. However, information about cytoskeletal proteins is still insufficient for determining cytoskeletal functions during reproduction, especially in terms of cell-cell interactions. One reason may be that cytological and biochemical research on the cytoskeleton of pollen and the embryo sac has not been complemented by sufficient research activity at genetic and molecular levels, and few laboratories are currently involved in this work. This might be because of problems in identifying appropriate applied applications of the work that might attract more investigation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Caffeine ; Freeze substitution ; Lilium ; Pollen tubes ; Rapid freeze fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In an attempt to correlate structural effects with the known dissipation of the tip-focused Ca2+ gradient caused by caffeine, we have examined the ultrastructure of caffeine-treated lily pollen tubes prepared by rapid freeze fixation and freeze substitution. We show that treatment with caffeine results in a rapid rearrangement of secretory vesicles at the pollen tube tip; the normal cone-shaped array of vesicles is rapidly dispersed. In addition, microfilament bundles appear in the tip region, where they had previously been excluded. Delocalized vesicle fusion continues in the presence of caffeine but tube extension ceases. Removal of caffeine from the growth medium initially causes tip swelling, delocalized vesicle fusion and presence of microfilaments well into the tip before normal structure and growth resume, concurrent with the previously reported return to a normal Ca2+ gradient.
    Type of Medium: Electronic Resource
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