ISSN:
0021-9304
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
The induction of tumor necrosis factor α (TNF-α) by polytetrafluoroethylene (PTFE) particles (5-50 μm) and by bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA) was examined in RAW cell cultures. Twenty-four-hour culture supernatants from the treated and control cells were assayed for TNF-α using a mouse L929 cell cytotoxicity assay. Untreated RAW cells produced low levels of endogenous TNF-α in the culture supernatants. Addition of 0.5 ng to 1 μg/ml LPS or 1 ng to 1 μg/ml LTA increased the TNF-α production by 7-3570-fold and 2-815-fold, respectively. Addition of 1-5 mg PTFE increased the TNF-α production by 6-17-fold over the untreated control cell levels. The cells exposed to PTFE and 0.5 ng/ml LPS or 5 ng/ml LTA produced TNF-α levels that were significantly higher than those produced by any inducer alone. Thus, both LTA, a Gram-positive bacterial cell wall component and LPS, a Gram-negative bacterial cell wall component, can induce TNF-α production, which is further enhanced by PTFE particles in RAW cells. © 1996 John Wiley & Sons, Inc.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
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