ISSN:
1435-604X
Keywords:
Carothenoporphyrin
;
Fluorescence ratio
;
Fluorescence spectroscopy
;
Tissue optics
;
Tumour detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Physics
,
Technology
Notes:
Abstract We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00592957
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