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  • 1995-1999  (3)
  • Colon cancer  (1)
  • Ergonovine  (1)
  • Fractal analysis  (1)
  • 1
    ISSN: 1432-2307
    Keywords: Key words Thrombospondin-1 ; p53 ; Colon cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  If activation of the p53 gene is involved in the progression or metastasis of colon cancer, it may affect the angiogenic phenotype in vivo. To verify this hypothesis, we studied the correlation between p53 accumulation and expression of thrombospondin-1 (TSP1) in colon cancer specimens. Levels of TSP1 gene expression were estimated by Northern blotting in 65 colon cancers. Accumulation of p53 and the distribution of TSP1 protein were evaluated immunohistochemically. Various levels of TSP1 gene expression were seen in colon cancers, while p53 accumulation was confirmed in 42 of the 65 colon cancers. The level of TSP1 gene expression demonstrated a significant inverse correlation with p53 accumulation in colon cancer. Colon cancer cells expressed TSP1 protein and p53 accumulation reciprocally in the same nests. These results suggest that alterations in the tumour suppressor gene p53 may inhibit TSP1 expression in colon cancer.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of wood science 45 (1999), S. 10-18 
    ISSN: 1611-4663
    Keywords: Multiresolutional contrast ; Fractal analysis ; Gloss of wood ; Texture analysis ; Digital image analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The gloss of wood is a unique texture compared to that of other materials. To express it quantitatively, two digital-image analyses were performed. One method was multiresolutional contrast analysis, which was the new method developed in this study. The other method was fractal image analysis. Twenty-four specimens, including solid woods, wood-plastic composites (WPCs), printed grains, and granites, were prepared. Digital images of specimens were obtained in five sizes and in two illuminant directions (perpendicular and parallel to the grain). The multiresolutional contrast values of perpendicular illuminated images were calculated and compared among specimens. The result of this quantitative analysis was that the gloss of wood was characterized by bright spots in a relatively small area. Using fractal analysis, the fractal dimension of a digital image was used as an index of brightness changes, not for expressing the self-similarity. These indices showed results similar to those of the multiresolutional contrast analysis.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1435-1803
    Keywords: Ergonovine ; coronary hyperconstriction ; serotonin2 receptor ; endothelium-dependent relaxation ; x-ray irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Mechanisms of ergonovine-induced coronary hyperconstriction were examined in vivo and in vitro in miniature pigs. To provoke coronary hyperconstriction, the endothelium of a segment of a major branch of the left coronary artery was denuded in 19 Göttingen miniature pigs (4 to 6 months of age). In Group I (n=12), the denuded site of the coronary artery was selectively irradiated with 15 Gy of x-ray twice, 3 and 4 months after endothelial denudation. The remaining 7 pigs were not irradiated (Group II). The vasoconstrictive effect of intracoronary administration of ergonivine (1 to 1000 μg) was examined angiographically 3 months (just before irradiation in group I) and 5 months after denudation in the two groups. After the angiographical study, the vessels were isolated and isometric tensions were measured in an organ chamber. In the in vivo studies, ergonovineinduced vasoconstriction at the denuded and x-ray irradiated site in Group I was significantly greater than that at the control site or that at the denuded site in Group II. Pretreatments with serotonin receptor blockers (ketanserin or methysergide) significantly attenuated ergonovine-induced hyperconstriction, while an alpha-adrenergic receptor blocker (prazosin) did not (% inhibition; ketanserin 74±9%, p〈0.01, methysergide 60±10%, p〈0.01, prazosin 9±5%, NS). In the in vitro studies, ergonivine produced significantly greater tension at the denuded and x-ray irradiated site (Group I) than at the control site or at the denuded site (Group II). Ergonovine-induced endotheliumdependent relaxation was impaired at the denuded site in both groups to a similar extent. These results suggest that ergonovine-induced hyperconstriction at the denuded and x-ray irradiated coronary artery resulted mainly from the hyperreactivity of medial smooth muscle mediated by serotonin2 receptors.
    Type of Medium: Electronic Resource
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