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  • 1995-1999  (3)
  • Cytokines  (1)
  • Integrin  (1)
  • Key words Phospholipases A  (1)
  • Schlüsselwörter Genitale weiße Piedra  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 288 (1996), S. 147-152 
    ISSN: 1432-069X
    Keywords: Key words Phospholipases A ; Epidermal inflammation ; Keratinocytes ; Cytokines ; Corticosteroids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phospholipase A 2 (PLA 2 )-catalysed liberation of arachidonic acid is the rate-limiting step in the generation of the lipid mediators prostaglandins and leukotrienes. PLA 2 regulation thus represents a pivotal mechanism in the pathogenesis of inflammation. In this study we investigated the effects of TNFα and IL-1α on PLA 2 activity in cultured murine keratinocytes. Starting 18 h after stimulation, PLA 2 activity increased significantly by about 250–320%) in the supernatants and in the cell pellets. This effect was completely inhibited either by preincubation of the cells with dexamethasone 48 h before stimulation or by coincubation with actinomycin D. PLA 2 acitivty detected in the supernatants was blocked by reduction with dithiothreitol, whereas the PLA 2 activity in the pellets was dithiothreitol-resistant. We conclude that in murine keratinocytes IL-1α induce de novo synthesis and release of a secretory PLA 2 and the induction of a different PLA 2 activity in the cytosol. These findings indicate a crucial link betweeen early cytokine effects and the initiation of the lipid mediator cascade in keratinocytes. The observation that PLA 2 induction could be completely inhibited by preincubation with dexamethasone allows new insights into the mechanism of steroid effects on epidermal inflammation and renders PLA 2 regulation an interesting therapeutic target.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 289 (1997), S. 194-203 
    ISSN: 1432-069X
    Keywords: Key words HMC-1 cell line ; Extracellular matrix ; protein ; Integrin ; c-kit ; Cadherin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Skin mast cells are typically located in the perivascular or perineural connective tissue. We observed that HMC-1 mast cells growing in suspension adhered efficiently to (〉 90% of cells) and spread on top of fibroblast monolayers and to a lesser degree on purified extracellular matrix proteins. Since adhesive interactions determine cell migration and tissue localization we studied the mechanism. It was found that HMC-1 cells attach to collagen I and fibronectin, laminin, collagen IV and vitronectin, but not to collagens III and VI or hyaluronic acid. Adhesion to fibronectin, collagen I and laminin was completely inhibited by mAbs blocking β 1 -integrins, whereas adhesion of HMC-1 cells to vitronectin was inhibited by anti-α v -chain mAbs. However, attachment of HMC-1 cells to fibroblasts was not influenced by mAbs blocking β 1 - or α v -chain function, by RGD peptides or by mAbs interfering with other receptors, most notably c- kit . Identical results were obtained with normal mast cells isolated from human foreskin. These results indicate that human mast cells attach to fibroblasts independently of β 1 - or α v -integrins as well as of c- kit receptor-mediated mechanisms. The functional characteristics observed (i.e. only partial sensitivity to trypsin and EDTA, no increase in trypsin sensitivity by pretreatment with EDTA) suggest that cadherin receptors were not involved, and it is likely that the adhesion process observed involved not-yet-defined heterotypic cell-cell adhesion receptors.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Der Hautarzt 47 (1996), S. 638-641 
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Genitale weiße Piedra ; Schambehaarung ; Trichosporon-Spezies ; Key words Genital white piedra ; pubic hair ; Trichosporon sp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary White piedra is a rare hair infection of man and certain animals, caused by the widespread Trichosporon fungi. The infection is characterized clinically by whitish, soft and easily removable coating of the hair shafts; however, the shafts may be destroyed by the pathogens invading at localized points. While all body hair may be affected, the pubic hair is most frequently affected. This paper is the first report genital white piedra in a 34-year-old Central European man. In all, 22 cases have been described world-wide in young men ranging in age from 20 to 40 years. Only 2 men, aged 42 and 53 years, do not fall in this range. Recently, a major African study disclosed this disease in women for the first time. In moderate latitudes, however, this infection appears to occur almost exclusively in young men.
    Notes: Zusammenfassung Die weiße Piedra ist eine seltene Haarerkrankung des Menschen und bestimmter Tiere durch die weitverbreitete Hefe der Gattung Trichosporon. Klinisch ist die Infektion durch weißliche, weiche und leicht abstreifbare Beläge an den Haarschäften charakterisiert; an umschriebenen Stellen können die Schäfte jedoch auch durch Invasion der Erreger zerstört werden. Prinzipiell können alle Körperhaare befallen sein; mit 95% am weitaus häufigsten ist jedoch die Genitalbehaarung betroffen. Wir berichten hier zum ersten Mal über eine genitale Piedra alba bei einem 34jährigen Mann aus dem deutschsprachigen Raum. Weltweit wurden bisher 22 Fälle bei jungen Männern im 3. und 4. Lebensjahrzehnt beschrieben. Nur 2 Männer mit 42 respektive 53 Jahren fallen aus dieser Altersverteilung heraus. Kürzlich wurde erstmals in einer großen afrikanischen Studie über diese Erkrankung detailliert auch bei Frauen berichtet. In den gemäßigten Breiten scheint diese Erkrankung jedoch fast ausschließlich bei jungen Männern vorzukommen.
    Type of Medium: Electronic Resource
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