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  • 1
    Electronic Resource
    Electronic Resource
    Enantiomeric separation of trimetoquinol, denopamine and timepidium by capillary electrophoresis and HPLC and the application of capillary electrophoresis to the optical purity testing of the drugs (1995)
    Springer
    Chromatographia 40 (1995), S. 638-644 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Capillary electrophoresis ; Enantiomer separation ; Trimetoquinol ; Denopamine ; Timepidium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Two methods for the direct separation of the enantiomers of trimetoquinol hydrochloride, denopamine and timepidium bromide were investigated, namely capillary zone electrophoresis (CZE) using cyclodextrins (CDs) (CD-CZE) and high-performance liquid chromatography (HPLC) using chiral stationary phases (CSPs). By CZE, separation of the enantiomers of trimetoquinol was successfully achieved when using β-cyclodextrin (β-CD), heptakis(2,6-di-0-methyl)-β-cyclodextrin (DM-β-CD) and β-CD polymer, but direct HPLC separation of the enantiomers was not successful. The enantiomers of denopamine were successfully separated by both CZE, employing DM-β-CD under acidic conditions, and HPLC, with cyclodextrin immobilized CSPs (CD-CSPs). Timepidium bromide, which is a racemic drug, was successfully enantioseparated by both CD-CZE and HPLC with CD-CSPs. The CD-CZE mode was selected as the optical purity testing method for trimetoquinol and denopamine because of the superiority of capillary electrophoretic (CE) techniques. Method validation results obtained clearly show that the CE method would be useful as an quality control method for assessing the optical purity of the drugs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02315130
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Capillary gel electrophoresis of oligonucleotides using polymer solutions (1998)
    Springer
    Chromatographia 48 (1998), S. 569-575 
    Details
    ISSN: 1612-1112
    Keywords: Capillary electrophoresis ; Oligonucleotides ; Dextran ; Polymer solution ; Purity testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Capillary gel electrophoresis (CGE) has been recognized as an effective method for the analysis of oligonucleotides. CGE using polymer solutions is especially useful and effective compared with that using crosslinked gels, because of easy change of media. Replacement of media leads to the reproducible separation of analytes. We have investigated CGE analysis of oligonucleotides of less than 20 bases employing various kinds of polymers. Polyacrylamide, dextrin, dextran, pullakin, and poly(ethylene glycol) were used as sieving matrixes at concentrations of 0–30 %. Polydeoxythymidylic acids [p(dT)11–20] were used as a test sample. These small oligonucleotides were successfully resolved on the basis of their base number by CGE using some of these polymer solutions. In particular, dextran was found to be effective and baseline separation was observed when a 30 % dextran solution was employed. Some validations such as linearity and reproducibility were also established and this method was found to be an adequate quality control method for small oligonucleotides. Finally, CGE using a 30 % dextran solution was successfully applied to impurity profiling of some synthetic oligonucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02466651
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Enantiomer separation by capillary electrophoresis using DEAE-dextran and aminoglycosidic antibiotics (1996)
    Springer
    Chromatographia 43 (1996), S. 426-430 
    Details
    ISSN: 1612-1112
    Keywords: Capillary electrophoresis ; Aminoglycosidic antibiotics ; DEAE-dextran ; Drugs ; Enantiomer separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Diethylaminoethyl (DEAE) dextran hydrochloride and three kinds of aminoglycosidic antibiotics; fradiomycin sulfate, kanamycin sulfate and streptomycin sulfate, were employed as chiral selectors in capillary electrophoresis, enantiomer separation. These selectors are cationic or basic because of amino functionality and therefore used for enantiomer separation of acidic compounds. To avoid adsorption of the basic or cationic selectors on the capillary inner surface, a coated capillary was employed. Among those tested, enantiomers of binaphthyl compounds and synthetic intermediates of diltiazem analogues were separated. Methanol addition was effective for the improvement of peak shape and resolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02271023
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    Paper (German National Licenses)
    Fulltext
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