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  • 1
    Electronic Resource
    Electronic Resource
    Unexpected DNA content in the endosperm of Cupressus dupreziana A. Camus seeds and its implications in the reproductive process (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 148-152 
    Details
    ISSN: 1432-2145
    Keywords: Key words Cupressus dupreziana ; DNA content ; Endosperm ; Reproduction ; Flow cytometry ; Gymnosperm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Nuclear DNA content of embryo and endosperm from mature and immature Cupressus dupreziana A. Camus seeds was estimated using laser flow cytometry. Relative DNA content of endosperm nuclei corresponded to four ploidy levels: 2C, 4C, 6C and 8C. The embryo nuclei invariably exhibited a diploid pattern. In all endosperm tissue analyzed no haploid nucleus was found. This is problematic since, in gymnosperms, endosperm and female gametes originate from one functional haploid megaspore produced by meiosis. The possible origin and derivation of C. dupreziana endosperm are discussed in light of previous results concerning the two other Mediterranean cypresses, C. sempervirens and C. atlantica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050132
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Flow cytometric evidence for multiple ploidy levels in the endosperm of some gymnosperm species (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 865-870 
    Details
    ISSN: 1432-2242
    Keywords: Key words Gymnosperms ; Ploidy ; Endosperm ; Embryo ; Flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Considered to be haploid tissue, the endosperm of coniferous trees has been extensively used by forest geneticists. Using laser flow cytometry, we show that endosperm ploidy level depends on the systematic position. The Abies, Cedrus and Pinus species tested exhibited uniform haploid endosperm compared to the diploid DNA content of the corresponding embryo. Endosperm of Cupressaceae contained multiple ploidy levels: Cupressus arizonica, Juniperus oxycedrus and Thuja orientalis endosperms exhibited a mixture of haploid–diploid nuclei, while C. atlantica and C. sempervirens endosperms contained six ploidy levels: 1C, 2C, 3C, 4C, 5C and 6C. Physiological and genetic implications of this original feature are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050488
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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