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Structural and immunochemical identification of Leb glycolipids in the plasma of a group O Le(a-b-) secretor (1995)

Henry, Stephen M. ; Jovall, Per-Åke ; Ghardashkhani, Sohbat ; [et al.]

Springer

Glycoconjugate journal 12 (1995), S. 309-317

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ISSN: 1573-4986

Keywords: Lewis negative ; Lewis antigens ; secretor ; plasma ; H type 1 ; mass spectrometry ; nuclear magnetic resonance spectroscopy

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract Total non-acid glycosphingolipids were isolated from the plasma of a healthy red blood cell group O Le(a-b-) salivary ABH secretor individual. Glycolipids were fractionated by HPLC and combined into eight fractions based on chromatographic and immunoreactive properties. These glycolipid fractions were analysed by thin-layer chromatography and tested for Lewis activity with antibodies reactive to the type 1 precursor (Lec), H type 1 (Led), Lea and Leb epitopes. Fractions were structurally characterized by mass spectrometry (EI-MS and LSIMS) and proton NMR spectroscopy. Expected blood group glycolipids, such as H type 1, (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glcβ1-1Cer) were immunochemically and structurally identified. Inconsistent with the red cell phenotype and for the first time, small quantities of Leb blood group glycolipids (Fucα1-2Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4Glcβ1-1Cer) were immunochemically and structurally identified in the plasma of a Lewis-negative individual. These findings confirm recent immunological evidence suggesting the production of small amounts of Lewis antigens by Lewis negative individuals.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00731334

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Liquid chromatography “on-flow” 1H nuclear magnetic resonance on native glycosphingolipid mixtures together with gas chromatography/mass spectrometry on the released oligosaccharides for screening and characterisation of carbohydrate-based antigens from pig lungs (1999)

Bäcker, Annika E. ; Thorbert, Sara ; Rakotonirainy, Olivier ; [et al.]

Springer

Glycoconjugate journal 16 (1999), S. 45-58

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ISSN: 1573-4986

Keywords: gas chromatography-mass spectrometry ; glycosphingolipids ; liquid chromatography -NMR spectroscopy ; pig ; xenotransplantation

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract Glycosphingolipids were prepared from pig lung and pooled into two fractions with (i) ≥3 sugar residues, and (ii) ≤3 sugar residues. Oligosaccharides were prepared and used for gas chromatography, gas chromatography-mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry. The glycolipid fractions i and ii were further characterised and purified using a novel method based on high performance liquid chromatography “on-flow” proton nuclear magnetic resonance. The LC “on-flow” NMR technique showed good chromatographic separation and gave NMR spectral information which could be used as guidance for pooling of the separated mixture glycolipids. Conventional 1H NMR, thin layer immunostaining, gas chromatography, gas chromatography/mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry were used to characterise the glycolipids and to validate LC-NMR spectral data.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006901803636

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