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  • 1
    Electronic Resource
    Electronic Resource
    Endochitinase and β-1,3-glucanase genes are developmentally regulated during somatic embryogenesis inPicea glauca (1997)
    Springer
    Planta 201 (1997), S. 189-194 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Endochitinase ; Fungal elicitation ; β-1,3-Glucanase ; Picea glauca ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNAs isolated from white spruce [Picea glauca (Moench) Voss] somatic embryos, are predicted to encode a basic class IV chitinase and a β-1,3-glucanase, respectively corresponding to genesPgChi-1 andPgGlu-1. Each represents a multigene family in spruce. Transcripts homologous toPgChi-1 orPgGlu-1 genes were highly abundant in embryogenic tissues and gradually decreased after tissues were placed on abscisic acid-containing maturation medium, with lowest abundance in globular embryos. Transcripts related toPgGlu-1 became highly abundant again in early cotyledonary embryos but decreased thereafter, whereas transcripts related toPgChi-1 were also highly abundant in late cotyledonary embryos and plantlets in vitro; transcripts were either low (PgChi-1) or were not detectable (PgGlu-1) in needles. Wounding, drying and flooding stresses enhancedPgChi-1-andPgGlu-1-related gene expression. Fungal cell wall suspension enhancedPgGlu-1-related transcript accumulation, but reducedPgChi-1-related transcript abundance within 24 h.PgChi-1 andPgGlu-1 and their homologues may have roles in plant defense, and possibly developmental roles during spruce somatic embryo maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007703
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  • 2
    Electronic Resource
    Electronic Resource
    A reliable method for extraction of RNA from various conifer tissues (1996)
    Springer
    Plant cell reports 15 (1996), S. 516-521 
    Details
    ISSN: 1432-203X
    Keywords: conifer ; Picea glauca ; RNA extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 μg up to 700 μg total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232985
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and characterization of six embryogenesis-associated cDNAs from somatic embryos of Picea glauca and their comparative expression during zygotic embryogenesis (1999)
    Springer
    Plant molecular biology 39 (1999), S. 859-864 
    Details
    ISSN: 1573-5028
    Keywords: embryo-abundant cDNAs ; gene expression ; gymnosperm ; Picea glauca ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Six somatic embryogenesis-associated cDNAs (PgEMB2, 6, 7, 8, 24 and 34) from white spruce (Picea glauca (Moench) Voss) somatic embryos have been characterized. Transcript accumulation during somatic embryo development and subsequent germination related to these genes, indicated that they were developmentally regulated. The transcripts related to clones PgEMB2, 6, 24 and 34 were also detected during zygotic embryo development, but transcripts of clones PgEMB7 and 8 were not. PgEMB24 had a similar gene expression pattern to spruce Em-like late embryo abundant (lea) gene, but other clones had no similarities in gene expression to either spruce lea-like or storage protein genes. Abscisic acid, a stimulator for spruce somatic embryo maturation, did not obviously affect gene expression corresponding to these cDNAs. The predicted proteins are distinguishable from known LEA proteins based on analyses of hydropathy plots, amino acid compositions and deduced protein structures. The similarities of the spruce cDNAs, and protein sequences predicted from these cDNAs, to other sequence data are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006146622614
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  • 4
    Electronic Resource
    Electronic Resource
    Transfer constants from complete molecular weight distributions (1997)
    Weinheim : Wiley-Blackwell
    Macromolecular Chemistry and Physics 198 (1997), S. 663-663 
    Details
    ISSN: 1022-1352
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1997.021980237
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  • 5
    Electronic Resource
    Electronic Resource
    Transfer constants from complete molecular weight distributions (1996)
    Weinheim : Wiley-Blackwell
    Macromolecular Chemistry and Physics 197 (1996), S. 403-412 
    Details
    ISSN: 1022-1352
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Transfer constants to chain-transfer agent (CTA) and to monomer can be obtained by consideration of the complete molecular weight distribution, using the slope of a plot of the natural logarithm of number-molecular weight distribution against molecular weight, in the limit of high molecular weight and low radical flux (P. A. Clay, R. G. Gilbert, Macromolecules 28, 552 (1995)). This method is applied to the bulk polymerisations of methyl methacrylate (MMA) with added triethylamine (TEA) and with added tert-dodecylmercaptan (mixture of 2,4,4,6,6-pentamethylheptane-2-thiol and 2,2,4,6,6-pentamethylheptane-4-thiol, TDM). The transfer rate coefficients are found to be 74 (±20) dm3 · mol- · s-l at 60°C for TEA, and 56 (±13) dm3 · mol-1 · s-1 at 25°C for TDM.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1996.021970131
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