ISSN:
1432-203X
Keywords:
Key words Malus domestica Borkh.
;
Microspore embryogenesis
;
Starvation
;
Cold treatment
;
Induction medium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We report, for the first time, the induction of embryogenesis and plant formation from isolated apple (Malus domestica Borkh.) microspores in vitro. Different isolation techniques were tested and an optimized protocol was elaborated. Furthermore, the influence of the induction medium and starvation treatment, using different starvation material, temperatures and time, were studied. In addition to embryo induction, the number of multicellular structures per divided microspores was found to be a suitable parameter of assessment and could be used in earlier stages during microspore culture. Although the number of embryos induced in these first experiments is low, the best frequency of embryo induction was shown to be at least twice as efficient as that obtained by anther culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050700
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