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  • 1995-1999  (2)
  • Reactivities of chymotrypsin  (1)
  • Toxicology  (1)
  • Menschliches Gewebe
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 109 (1996), S. 42-44 
    ISSN: 1437-1596
    Keywords: Toxicology ; Drowning ; Sulfides ; Human tissue ; Distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract An adult male was found dead beneath a pool of sewage in the pump room of a fish market. Autopsy revealed the cause of death to be suffocation after aspirating sewage into the respiratory tract. Since hydrogen sulfide gas was detected in the atmosphere at the scene of the accident, gas poisoning was suspected and toxicological analysis of sulfides in body tissues was performed. The concentrations of sulfides in the blood, lung and kidney were 0.95 μmol/ml, 0.22 and 0.38 μmol/g, respectively. These values were remarkably higher than those in previously reported cases involving exposure to hydrogen sulfide gas. Therefore, oral intake of sulfides was assumed and the distribution of sulfides in tissues following oral administration of sodium sulfide solution was examined by means of animal experiments using rats. The concentration of sulfides in the blood from rats following oral intake was much higher than that seen following gas exposure. Based on these results, we concluded that the victim had been exposed to hydrogen sulfide gas and had then collapse into a pool of sewage containing sulfides. The sulfides which were distributed throughout the body tissues had mainly issued from the alimentary tract prior to death by drowning.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Colloid & polymer science 106 (1997), S. 257-261 
    ISSN: 1435-1536
    Keywords: Reactivities of chymotrypsin ; gelation of alginate ; immobilized enzyme ; effects of gelation ; capsule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract To elucidate adding effects of alginate (Alg) and its gelation on the reactivities of chymotrypsin (Chy), three kinds of Chy-immobilized capsules were prepared by an interfacial condensation, i.e., (a) a capsule containing only Chy in the core (Chy capsule), (b) a capsule containing Chy in NaAlg (Chy capsule(NaAlg)) and (c) a capsule containing Chy in CaAlg gel (Chy capsule(CaAlg)). The coated film was poly(ethylenediamine-terephthaloyl) (PEDT) and NaAlg in the core was gelled in the state adhered tightly to the PEDT film. These Chyimmobilizing capsules were found to be useful for determining the effects of the gelation around Chy. 4-nitrophenyl acetate was used as a substrate. From the rates of efflux of the product (p-nitrophenol), apparent maximum velocitiesv max and the apparent Michaelis constantsK′m were obtained. The values ofv′max showed the maximum at pH=7.8 for all three capsules and were found not to show any pH shift. The magnitude ofv′max was in the following order: Chy capsule (CaAlg) 〉 Chy capsule (NaAlg)-Chy capsule. The adding effects of Alg and its gelation on the reactivities of Chy are discussed.
    Type of Medium: Electronic Resource
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