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  • 1
    Electronic Resource
    Electronic Resource
    Nuclear and cytosolic calcium levels in NIH 3T3 fibroblasts (1997)
    Springer
    Experimental biology online 2 (1997), S. 1-9 
    Details
    ISSN: 1430-3418
    Keywords: Cytosolic calcium ; Image analysis ; NIH3T3 fibroblasts ; Nuclear calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The spatial distribution of intracellular calcium in resting NIH 3T3 fibroblasts loaded with Fura-2 has been studied by digital image analysis. Calibration parameters were determined separately for the nucleus and the cytosol to take into account possible differences in the physico-chemical properties of the two compartments and were found not to differ significantly. The apparent resting calcium concentration in these cells was found to be significantly lower in the nucleus than in the cytoplasm; however, this difference appears to be an artefact arising from the presence in the cytoplasm of regions with higher calcium levels. Application of thapsigargin, to block active uptake of calcium into these compartments, substantially eliminated the differences between nuclear and cytosolic calcium concentrations. These observations indicate that nuclear and cytosolic calcium are in equilibrium in the resting fibroblasts and argue against the existence of diffusional barriers between these two compartments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00898-997-0009-4
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dietary spermine supplementation induces intestinal maturation in sea bass (Dicentrarchus labrax) larvae (1997)
    Springer
    Fish physiology and biochemistry 16 (1997), S. 479-485 
    Details
    ISSN: 1573-5168
    Keywords: polyamine ; spermine ; sea bass ; larvae ; intestinal maturation ; pancreatic enzymes ; intestinal enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sea bass (Dicentrarchus labrax) larvae were fed microparticulated compound diet containing 0 (FP0), 0.10 (FP10) and 0.33% (FP33) of a polyamine, spermine, from day 20 to day 38. LP group was fed live prey. This group exhibited the highest growth and survival. The addition of spermine did not lead to growth enhancement. A 33% survival improvement was obtained in FP33 group compared to FP0 group. The spermine addition affected the activity of pancreatic enzymes, trypsin, chymotrypsin and amylase, during larvae development. This non specific effect suggested that the action of spermine would be mediated by hormones. In the intestine, the FP33 group exhibited from day 31 higher activities of brush border membrane enzymes (leucine aminopeptidase and alkaline phosphatase) and lower level in a cytosolic enzyme (leucine-alanine peptidase) compared to FP10 and FP0 group. The diet containing the highest spermine level induced an enzymatic profile similar to that obtained in LP group and characteristic of a mature enterocyte. The initiation of enterocyte maturation at a proper development stage was associated to the survival improvement observed in FP33 group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007786128254
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    Paper (German National Licenses)
    Fulltext
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