ISSN:
1432-1335
Keywords:
Key words Hepatocellular carcinoma (HCC)
;
Targeting therapy
;
Single-chain antibody
;
Immobilized metal chelate affinity chromatography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Monoclonal antibodies have been widely used in tumor targeting studies with promising results. However, their clinical application has been limited by heterogeneity and macro-molecular movement of murine antibody. In this study, the variable-region (heavy- and light-chain) fragments of anti-HBx monoclonal antibody were enriched by the polymerase chain reaction. The expression vector, which included a 6x histidine sequence in the 3′ terminus of the HBx single-chain antibody (sFv) was recombined with a linker sequence (KLGGGGFSGA) between the variable regions. The expression product from Escherichia coli fused with 6xHis was purified by nickel (Ni2+) nitrilotriacetate chelating resin. The results of enzyme-linked immunosorbent assay and Western blotting showed that sFv had binding affinity with HBxAg, suggesting that it could become a novel targeting carrier in clinical trials.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004320050113
Permalink
