ISSN:
1432-1335
Keywords:
Gastric carcinoma
;
p53 Immunohistochemistry
;
PCR-SSCP analysis
;
TP53 mutations
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract TP53 gene mutations, one of the most common alterations described in human tumors, have also been detected in gastric carcinoma, and shown to occur rather late in disease progression. A better assessment of the prognostic value of TP53 gene mutations can be obtained by examining archival material, as this allows stored cases with well-defined histories to be monitored. We performed immunohistochemical and polymerase chain reaction/single-strand comformation polymorphism (PCR-SSCP) analyses of formalin-fixed paraffin-embedded material from nine selected cases of gastric carcinoma at different pathological stages. PCR-SSCP analysis of TP53 exons 5–8 detected missense point mutations in two out of five immunostain(PAb1801)-positive tumors, and a deletion (allowing for a premature stop codon) in one of the remaining four immunostain-negative tumors. Thus, PCR-SSCP analysis represents a feasible strategy for the detection of TP53 alterations in archival material of gastric carcinoma cases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01202217
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