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  • 1995-1999  (2)
  • Vibrio  (1)
  • genetic differentiation  (1)
  • 1
    Electronic Resource
    Electronic Resource
    NH4 + transport system of a psychrophilic marine bacterium, Vibrio sp. strain ABE-1 (1999)
    Springer
    Extremophiles 3 (1999), S. 89-95 
    Details
    ISSN: 1433-4909
    Keywords: Key words Psychrophilic bacterium ; Vibrio ; NH4+ transport system ; 14CH3NH3+ uptake ; Nitrogen source for growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract NH4 + transport system of a psychrophilic marine bacterium Vibrio sp. strain ABE-1 (Vibrio ABE-1) was examined by measuring the uptake of [14C]methylammonium ion (14CH3NH3 +) into the intact cells. 14CH3NH3 + uptake was detected in cells grown in medium containing glutamate as the sole nitrogen source, but not in those grown in medium containing NH4Cl instead of glutamate. Vibrio ABE-1 did not utilize CH3NH3 + as a carbon or nitrogen source. NH4Cl and nonradiolabeled CH3NH3 + completely inhibited 14CH3NH3 + uptake. These results indicate that 14CH3NH3 + uptake in this bacterium is mediated via an NH4 + transport system and not by a specific carrier for CH3NH3 +. The respiratory substrate succinate was required to drive 14CH3NH3 + uptake and the uptake was completely inhibited by KCN, indicating that the uptake was energy dependent. The electrochemical potentials of H+ and/or Na+ across membranes were suggested to be the driving forces for the transport system because the ionophores carbonylcyanide m-chlorophenylhydrazone and monensin strongly inhibited uptake activities at pH 6.5 and 8.5, respectively. Furthermore, KCl activated 14CH3NH3 + uptake. The 14CH3NH3 + uptake activity of Vibrio ABE-1 was markedly high at temperatures between 0° and 15°C, and the apparent K m value for CH3NH3 + of the uptake did not change significantly over the temperature range from 0° to 25°C. Thus, the NH4 + transport system of this bacterium was highly active at low temperatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050103
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Allozyme variation and behavioural dimorphism among populations of Littorina brevicula (Philippi) from Japan (1998)
    Springer
    Hydrobiologia 378 (1998), S. 53-57 
    Details
    ISSN: 1573-5117
    Keywords: Littorina brevicula ; migration behaviour ; genetic differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Amakusa (Japan), an apparently homogeneous population of Littorina brevicula, divides into two groups during the reproductive season (winter). Some snails migrate to the lower intertidal zone, while the rest remain in the upper zone. This migration behaviour might be controlled genetically and the behavioural dimorphism may be maintained by assortative mating. To evaluate this hypothesis, we studied genetic variation at four polymorphic enzyme loci (Pgm, Aldh, Ipp, Gpt) in both groups, as well as in seven other samples from the coast of Japan and Vostok Bay (Russian coast of the Sea of Japan). There were no significant allele frequency differences between the two behavioural groups of snails with differing migration patterns (χ2 = 8.411, df = 12, p 〉 0.5). The samples from Japan belong to a panmictic population. Yet, a significant heterogeneity was found between the sample from Vostok Bay and the samples from Japan, indicating a reduced gene flow between these regions. Generally, observed genotype frequencies showed good fits to Hardy-Weinberg equilibrium expectations at three of the four loci investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003225218693
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    Paper (German National Licenses)
    Fulltext
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