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Descriptive parameter evaluation in mammalian cell culture (1998)

Dutton, Roshni L. ; Scharer, Jeno M. ; Moo-Young, Murray

Springer

Cytotechnology 26 (1998), S. 139-152

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ISSN: 1573-0778

Keywords: batch kinetics ; cell cycle ; cell-hours ; hybridoma ; population parameters ; productivity

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract Several methods exist for assessing population growth and protein productivity in mammalian cell culture. These methods were critically examined here, based on experiments with two hybridoma cell lines. It is shown that mammalian cell culture parameters must be evaluated on the same basis. In batch culture mode most data is obtained on a cumulative basis (protein product titre, substrate concentration, metabolic byproduct concentration). A simple numerical integration technique can be employed to convert cell concentration data to a cumulative basis (cell-hours). The hybridoma lines used in this study included a nutritionally non-fastidious line producing low levels of MAb and a nutritionally fastidious hybridoma with high productivity. In both cases the cell-hour approach was the most appropriate means of expressing the relationship between protein productivity and cell population dynamics. The cell-hour approach could be used as the basis for all metabolic population parameter evaluations. This method has the potential to be used successfully for both prediction and optimization purposes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007940119503

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Influence of the gas - liquid separator design on hydrodynamic and mass transfer performance of split-channel airlift reactors (1995)

Choi, Keun H. ; Chisti, Yusuf ; Moo-Young, Murray

Chichester : Wiley-Blackwell

Journal of Chemical Technology AND Biotechnology 62 (1995), S. 327-332

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ISSN: 0268-2575

Keywords: airlift reactor ; gas-liquid separator ; hydrodynamics ; oxygen transfer ; wastewater treatment ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Three geometric configurations of gas-liquid separators were used in split-channel airlift reactors (0·1 m3 liquid volume; riser-to-downcomer cross-sectional area ratio = 0·7; aspect ratio = 3.6) to test the effect of geometry on hydrodynamic performance and oxygen transfer behaviour. For otherwise fixed conditions, the design of gas-liquid separators affected the induced liquid circulation rate, the depth of penetration of the bubble layer in the downcomer, the gas holdup in the downcomer, the mixing time and the overall volumetric gas-liquid oxygen transfer coefficient. The gas holdup in the riser was only marginally affected by the design of the separator. The impact of the various separator designs on hydrodynamic behaviour could be explained as emanating from a combination of the gas-liquid separating ability of the design and its hydraulic resistance.

Additional Material: 7 Ill.