ISSN:
1573-7330
Keywords:
bovine IVM/IVF/IVC blastocysts
;
laser zona pellucida drilling
;
embryo hatching
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Purpose: Our purpose was to test whether zona pellucida (ZP) drilling using a 1.48-μm diode laser beam on bovine IVM/IVF/IVC blastocysts is effective for embryo hatching. Methods: Blastocysts produced in vitro at day 7 after IVF were divided into control and laser-drilled groups, respectively. Results: When the rates of in vitro development of bovine embryos were examined, the average cleavage rate (〉two-cell) was 82.3% and the blastocyst rate at day 7 after IVF was 32.5%. Using these blastocysts, when the laser drilling effect was investigated at 48 hr after treatment, the total hatching rate in the laser-drilled group (98.0%) was significantly higher than that in the control group (60.0%)(P 〈 0.001). Especially, the hatched rate of the laser-drilled group (68.0%) was significantly enhanced compared with that of the control group (30.0%) (P 〈 0.001). Conclusions: These results demonstrated that laser ZP drilling on bovine IVM/IVF/IVC blastocysts can significantly increase the hatching rate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022573024170
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