ISSN:
1573-6903
Keywords:
Piperine
;
cerebellar granule neurons
;
neuronal death
;
low K+medium
;
apoptosis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We compared neurotoxicity of piperine and low K+on cultured cerebellar granule neurons. As considered from lactate dehydrogenase release and 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide reduction, both piperine and shifting from high K+(25 mM) to low K+(5.4 mM) were cytotoxic to cerebellar granule neurons. Protein synthesis inhibitors, cycloheximide and anisomycin, and an endonuclease inhibitor, aurintricarboxylic acid, were protective against low K+-induced neuronal death whereas they were ineffective against that induced by piperine. D-α-tocopherol, trolox, and a spin trap 3,3,5,5-tetramethyl-l-pyrroline-l-oxide were protective against piperine neurotoxicity whereas they had no effect on that induced by low K+. These results suggest that piperine and low K+may exert neurotoxic effects on cerebellar granule neurons through different mechanisms. Death of cerebellar granule neurons induced by piperine may be mediated by non-apoptotic mechanisms and may involve membrane lipid peroxidation and/or free radical generation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022461706344
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