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  • 1
    ISSN: 1573-7330
    Keywords: karyotype analysis ; chromosomal anomaly ; preimplantation diagnosis ; fluorescence in situ hybridization ; spontaneous abortion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: Our purpose was to select the proper chromosomes for preimplantation diagnosis based on aneuploidy distribution in abortuses and to carry out a feasibility study of preimplantation diagnosis for embryos using multiple-probe fluorescence in situ hybridization (FISH) on the selected chromosomes of biopsied blastomeres. Methods: After determining the frequency distribution of aneuploidy found in abortuses, seven chromosomes were selected for FISH probes. Blastomeres were obtained from 33 abnormal or excess embryos. The chromosome complements of both the biopsied blastomeres and the remaining sibling blastomeres in each embryo were determined by FISH and compared to evaluate their preimplantation diagnostic potential. Results: Chromosomes (16, 22, X, Y) and (13, 18, 21) were selected on the basis of the high aneuploid prevalence in abortuses for the former group and the presence of trisomy in the newborn for the latter. Thirty-six (72%) of 50 blastomeres gave signals to permit a diagnosis. Diagnoses made from biopsied blasotmeres were consistent with the diagnoses made from the remaining sibling blastomeres in 18 embryos. In only 2 of 20 cases did the biopsied blastomere diagnosis and the embryo diagnosis not match. Conclusions: If FISH of biopsied blastomere was successful, a preimplantation diagnosis could be made with 10% error. When a combination of chromosome-13, -16, -18, -21, -22, -X, and -Y probes was used, up to 65% of the embryos destined to be aborted could be detected.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0867
    Keywords: biological N2 fixation ; Cajanus cajan L. Millsp. intercropping ; minirhizotron ; natural15N abundance ; pigeonpea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-0867
    Keywords: fertilizer nitrogen use efficiency ; intercropping ; N budget ; pigeonpea ; sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cropping systems and fertilizer management strategies that effectively use applied nitrogen (N) are important in reducing costs of N inputs. We examined the effect of time of N application on dry matter (DM) and grain yield (GY), N accumulation, the N budget in crop from soil, fertilizer and atmosphere, and the fertilizer N use efficiency (estimated by the conventional difference method, and the direct 15N recovery by the crops), in a sorghum/pigeonpea intercropping system on an Alfisol (Ferric Luvisols (FAO); or Udic Rhodustalf (USDA) in India. Fertilizer N was applied at planting (basal) and at 40 days after sowing (delayed). Nitrogen was applied only to the sorghum rows in the intercropping treatment. Nitrogen derived from air (Ndfa) was estimated by the15 N natural abundance method, and N derived from fertilizer (Ndff) was estimated by the 15N isotope dilution method. Delaying N fertilization till 40 days after sowing (DAS), rather than applying at sowing increased DM and GY of the sorghum, but not of pigeonpea. Delaying N fertilization to sorghum for 40 days significantly (p〈0.001) increased 15N recovery in shoot from 15 to 32% in sole crop, and from 10 to 32% in intercrop. Similarly, there was a significant (p〈0.001) increase in N recovery (by the difference method) from 43 to 59% in sole crop and from 28 to 71 % in intercrop sorghum. Fertilizer N recovery by sole crop pigeonpea (14%) was higher than intercrop pigeonpea (2–4%). Pigeonpea fixed between 120–170 kg ha-1 of atmospheric N throughout the cropping season. Although there was a marked difference in nitrate-N (N03-N) concentrations between basal and delayed treatments at planting, no difference was observed in N03-N concentrations in soil solution between the treatments at 40 DAS. Our data on N accumulation by plants showed that the rate of N depletion or disappearance from the soil solution was 2–3 times faster than N accumulation by plants, suggesting that an appreciable amount of N03-N would disappear from soil solution in the top soil without being utilized by crops during the initial growth stage.
    Type of Medium: Electronic Resource
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