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  • 1995-1999  (2)
  • river buffalo  (2)
  • 1
    ISSN: 1573-6849
    Keywords: cattle ; chromosome ; cosmid ; fluorescence in situ hybridization ; river buffalo ; synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three bovine cosmid-derived microsatellites (IDV-GA49, IDVGA7 and IDVGA47), previously assigned to cattle syntenic groups U1, U7 and U21, respectively, were fluorescence in situ hybridization (FISH) mapped to river buffalo (Bubalus bubalis, L., 2n= 50) chromosomes (BBU) 3p22 (IDVGA47, U21), BBU 5q21 (IDVGA49, U1) and BBU 5p19 (IDVGA7, U7) using sequential FISH and R-banding techniques. These localizations allowed the assignment, for the first time, of the bovine syntenic groups U21, U1 and U7 to specific river buffalo chromosomes. FISH mapping of IDVGA7 (U7) to cattle rob(1;29) p-arms confirms the banding homologies between BTA 29 and BBU 5p and further supports the idea that cattle standard karyotypes need adjustments.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6849
    Keywords: cosmid ; fluorescence in situhybridization ; nomenclature ; R-banding ; river buffalo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Six bovine cosmid-derived microsatellites (IDVGA53, BTA3/U6; IDVGA61, U13; IDVGA41, BTA12/U27; IDVGA32, BTA15/U19; IDVGA59, BTA26/U26 and IDVGA71, U8), previously assigned to cattle chromosomes, were FISH-mapped to river buffalo chromosomes (BBU) 6q15, 8q34, 13q15, 16q25, 23q22 and 24q13 respectively. Sequential FISH/RBA-banding allowed the precise identification of chromosomes and localization of probe-signals on chromosome bands. These localizations allowed us to assign indirectly, for the first time, six bovine syntenic groups to river buffalo chromosomes, thereby extending its physical map. The localization of IDVGA71 (bovine U8) to the marker BBU24 adds further information to resolve definitively cattle chromosome ambiguities involving cattle chromosomes 25, 27 and 29.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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