ISSN:
1040-0397
Keywords:
Biosensor
;
Graphite felt
;
Glucose oxidase
;
Lactate oxidase
;
Enzyme reactor
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Immobilization of glucose oxidase in conducting polymers electrochemically generated from pyrrole, N-methylpyrrole, aniline and ortho-phenylene diamine on platinum surfaces was used to construct amperometric glucose biosensors. High sensitivity was obtained with a sensor utilizing polymerized o-phenylene diamine as entrapment matrix. This polymer, as well as polypyrrole, was used to prepare a new type of enzyme reactors with immobilized glucose or lactate oxidase by depositing enzyme-polymer layers on pieces of graphite felt. Mounted into a column, the felt can then be applied as an enzyme reactor in a flow system. It can either be used as working electrode itself in an electrochemical detection system or be combined with a separate detector. A microelectrode array was chosen as an example of the latter. With this system, detection limits lower than 500 μM and a linear range up to 10 mM were obtained for glucose and a detection limit lower than 50 μM and a linear range up to I mM for lactate, respectively.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140081212
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