ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The degradation of 2-chloroethanol by Pseudomonas putida US 2 was investigated in shaking flasks, air-bubble columns and packed-bed fermenters by free cells, calcium-alginate-entrapped cells and on cells on granular clay adsorbed. Entrapped cells tolerated increasing concentrations of 2-chloroethanol better than free cells. Their maximum degradative activity could be observed at 34°C and pH 7.0. The degradation of 2-chloroethanol leads to a decrease of pH and to a stagnation of mineralization, particularly with free or entrapped cells. Following the stabilization of pH, supplementation with succinate resulted in a complete degradation of higher 2-chloroethanol concentrations. Less 2-chloroethanol was degraded in air-bubble columns and larger amounts in packed-bed fermenters. 2-Chloroethanol was mineralized faster by free or entrapped P. putida US 2 than by adsorbed cells, which, on the other hand, were able to remove higher concentrations of the compound. The results with P. putida US 2 are a good indication that this microorganism could be used in waste-water treatment and soil-decontamination systems.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002530050550
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