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  • 1995-1999  (3)
Source
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Electrotransformation of three pathovars of Xanthomonas campestris (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 651-655 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Procedures for electrotransformation have been adapted for three pathovars of Xanthomonas campestris: campestris, vesicatoria and manihotis. Three differently sized plasmids (51, 9.3 and 3.3 kb) at different concentrations (10, 30 and 50 ng/sample) and different field strengths (10, 12, 14 and 18 kV/cm) were used. The efficiency of transformation was dependent on the recipient strain and the plasmid introduced. In general, a field strength of 14 kV/cm as well as a concentration of 30 ng plasmid DNA/sample seemed to be adequate for most conditions. Only X. campestris pv. vesicatoria strain 479 required a higher field strength for better efficiency. The plasmid size was inversely related to the efficiency of transformation; up to 1.6×1010, 5.3×107 and 3.7×105 transformants/μg DNA were obtained using the pXG31 (3.3 kb), pUFR027 (9.3 kb) and RP4 (51 kb) plasmids, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00164769
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Electrotransformation of three pathovars of Xanthomonas campestris (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 651-655 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Procedures for electrotransformation have been adapted for three pathovars of Xanthomonas campestris: campestris, vesicatoria and manihotis. Three differently sized plasmids (51, 9.3 and 3.3 kb) at different concentrations (10, 30 and 50 ng/sample) and different field strengths (10, 12, 14 and 18 kV/cm) were used. The efficiency of transformation was dependent on the recipient strain and the plasmid introduced. In general, a field strength of 14 kV/cm as well as a concentration of 30 ng plasmid DNA/sample seemed to be adequate for most conditions. Only X. campestris pv. vesicatoria strain 479 required a higher field strength for better efficiency. The plasmid size was inversely related to the efficiency of transformation; up to 1.6 × 1010, 5.3 × 107 and 3.7 × 105 transformants/μg DNA were obtained using the pXG31 (3.3 kb), pUFR027 (9.3 kb) and RP4 (51 kb) plasmids, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00164769
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Nucleon time-like form factors below the $\mathrm{N}\overline{\mathrm{N}}$ threshold (1999)
    Springer
    The European physical journal 11 (1999), S. 709-715 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. The nucleon magnetic form factors in the unphysical region, i.e., for time-like $Q^2$ but below the N $\overline{\mathrm{N}}$ threshold, have been obtained by means of dispersion relations in a model-independent way, without any bias towards expected resonances. Space-like and time-like data have been employed, along with a regularization unfolding method to solve the integral equation. Remarkably, resonance structures with peaks for the $\rho (770)$ and $\rho^{\prime} (1600)$ and a structure near the $\mathrm{N} \overline{\!\mathrm{N}}$ threshold are automatically generated. The obtained $\rho$ has a much larger width, whose significance is explored. No evidence is found for a peak at the $\Phi$ mass, in spite of the expectation that such a peak exists when there is a sizeable polarized s $\overline{\mathrm{s}}$ content in the vector current of the nucleon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100529900077
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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