ISSN:
1573-5028
Keywords:
α-prolamin
;
kafirin
;
monocot gene expression
;
transgenic tobacco
;
zein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Sequences corresponding to 855 bp of 5′ promoter region and the transit peptide from ⋋GK.1, a genomic clone encoding a 22 kDa α-kafirin seed protein from sorghum, were translationally fused to a cloned β-glucuronidase (GUS) coding sequence from uidA and transferred to tobacco via Agrobacterium tumefaciens-mediated transformation. No GUS expression was detectable at any stage of growth in stems or leaves of these plants. However, GUS expression was detected in both embryo and endosperm tissues of resulting tobacco seeds 10–15 days after flowering. Dissected tissues indicate endosperm expression was localized within the bulk endosperm and not within the parenchyma cell layer underlying the integument. These studies also demonstrate that within dissected tobacco embryos, expression from the kafirin promoter was restricted to the mesocotyl region.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00041386
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