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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of the allergen Der f 7 from house dust mite extracts by species-specific and crossreactive monoclonal antibodies (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 27 (1997), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The group 7 mite allergens react with IgE in 50% of sera from allergic patients.Objective To determine the molecular and antigenic characteristics and heterogeneity of Der f 7 in mite extracts.Methods Monoclonal antibodies (MoAbs) produced from mice immunized with recombinant Der f 7 were examined for crossreactivity to Der p 7 and then used for immunoblotting of 1 and 2-D gel electrophoresis. Deglycosylation was studied with N-glycosidase-F and N-terminal sequencing by Edman degradation. The epitopes of the monoclonal antibodies were compared by cross-inhibitory immunoassays.Results Immunoblotting of D. farinae extracts with all the anti Der f 7 MoAbs showed major reactivities at 31, 30 and 25 kDa. The strongest immunostaining was at 25 kDa which contrasted with Der p 7 where the 31 and 30 kDa bands were strongest. The relative strength of staining however varied between extracts. The 31 and 30 kDa components were glycosylation products of the 25 kDa form which had the N-terminal sequence predicted from cDNA analysis. Two MoAbs stained an 18 kDa band consistent with a degradation product. The 2-D gels showed that different components with pls from 5.6–6.4. Both species-specific and Der p 7 crossreactive MoAbs were produced and a two-site ELISA assay for detecting group 7 allergen was developed with MoAbs recognizing different epitopes.Conclusions Der f 7 has been defined by its natural N-terminal sequence and MoAbs. It apparently exists as different glycosylation and degradation products in mite extracts, the relative abundance of which differs with different preparations. A two-site ELISA to measure the allergen was developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.1997.650890.x
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  • 2
    Electronic Resource
    Electronic Resource
    IgE and monoclonal antibody binding by the mite allergen Der p 7 (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 26 (1996), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The recently characterized group 7 house dust mite allergens give positive skin-test reactions in 53% of allergie patients.Objective This study was performed to compare the IgE bindinig activity of natural and recombinant Der p 7, to measure the binding in allergic sera in comparison to major allergen Der p 2 and characterize the response by competitive inhibition with monoclonal antibodies.Methods IgE anti Der p 2 and Der p 7 antibodies against the recombinant allergens and monoclonal binding activities were measured by a solid phase radioimmune assay. Reslts A competitive binding assay showed that rDer p 7 inhibited 91% of IgE-binding to natural Der p 7 in 2 sera and 73% in a further two. The IgE binding of rDer p 2 and Der p 7 from 41 sera was then compared. Of the sera 88% and 46% respectively showed positive binding. All of the 19 sera which bound Der p 7 also bound Der p 2 but 11 (58%) had bound IgE to Der p 7 as high or higher than the binding to Der p 2. These sera were mostly high responders to both allergens. A panel of six monoclonal antibodies produced against either rDer p 7 or rDer f 7 was used for epitope analysis. All of these reaeted with eaeh allergen by enzyme linked immunosorbent assay (ELISA) and immunoblotting. Two patterns of cross inhibition of monoclonal antibody binding were observed and of five monoclonal antibodies tested, lour could inhibit the binding of IgE (WH9, WH22. WPS and HD19) while one (WH14) could not.Conclusions Although Der p 7 only reacts with 50% of allergic sera it often has a high IgE binding activity and may be more important than the major Der p 2 allergen in a high percentage of subjects. The combined competitive inhibition experiments show the IgE response is directed at several specilieities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1996.tb00096.x
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  • 3
    Electronic Resource
    Electronic Resource
    Aquabis(2,3-butanedione dioximato-N,N')(cyclopentyl)cobalt(III) (1998)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 1823-1825 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270198009068
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  • 4
    Electronic Resource
    Electronic Resource
    Periodicity in midwest US climatic data: the Hale cycle signal or the luni-solar signal? (1998)
    Springer
    Stochastic environmental research and risk assessment 12 (1998), S. 205-222 
    Details
    ISSN: 1436-3259
    Keywords: Key words: Hale cycle ; luni-solar tidal constituent ; maximum entropy spectrum ; multi-taper method ; harmonic analysis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract: Cohen and Sweeter (1975) found the 20 to 22-year Hale (double sunspot) cycle signal in the maximum entropy spectra of sunspot and Atlantic tropical cyclone data. Currie (1996) corroborated and extended their analysis, but he argued that this signal is the 18.6-year luni-solar tidal constituent. Currie maintains that Cohen and Sweeter mistakenly conjectured that this long term periodicity was induced by the 20 to 22-year Hale cycle signal. However, no further investigation of periodicity in the extracted wave forms corresponding to the 18.6-year luni-solar signal was conducted. In this study, we follow Currie's signal processing procedures to extract the wave forms corresponding to the 18.6-year luni-solar signal. In order to investigate the periodicity in the extracted wave forms, multi-taper method (MTM) is used for harmonic analysis. Band pass filters are then designed to extract the wave forms corresponding to the individual components identified in the MTM harmonic analysis. The investigation results of the monthly precipitation and Palmer's drought severity index (PDSI) data in three of the midwestern states – Illinois, Indiana and Ohio – show that two periodic components, the 20 to 22-year Hale cycle signal and the other component with periods between 16.9 and 13.5 years, are identified. The bistability phenomenon, which Currie found in these wave forms, is more likely to result from the superposition of these two periodic components, rather than from a nonlinear mechanism. Besides, a periodic component with an approximate period of 33 years is detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004770050018
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  • 5
    Electronic Resource
    Electronic Resource
    Isozymes of nuclear protein tyrosine kinase during chemical induced hepatocarcinogenesis in rats (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 527-529 
    Details
    ISSN: 1432-1335
    Keywords: Nuclear protein tyrosine kinase ; Hepatocarcinogensis ; Isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The activity changes of isozymes of nuclear protein tyrosine kinase (nPTK) during chemically induced hepatocarcinogenesis in rats was studied. Two isozymes were isolated from rat liver by using ion exchange chromatography. These two isozymes were designated as nPTK-I and nPTK-II, and were found to be minor and major components of total nPTK respectively. These proved to be two different enzymes due to their different characteristics, such as molecular weights, electrical charges and kinetics. The specific activity nPTK-I and its percentages in total nPTK significantly increased in preneoplastic stage (week 10) and slightly elevated further in neoplastic stage (week 18). The specific activity of the nPTK-II moderately increased at week 10, but compared to its value at week 10, it decrease at week 18, resulted in gradual decrease of its percentage in total nPTK. These results indicate that rat hepatic nPTK-I and nTPK-II may be related to neoplastic and preneoplastic stages of rat liver respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01192209
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  • 6
    Electronic Resource
    Electronic Resource
    Isozymes of nuclear protein tyrosine kinase during chemical induced hepatocarcinogenesis in rats (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 527-529 
    Details
    ISSN: 1432-1335
    Keywords: Key words Nuclear protein tyrosine kinase ; Hepatocarcinogensis ; Isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The activity changes of isozymes of nuclear protein tyrosine kinase (nPTK) during chemically induced hepatocarcinogenesis in rats was studied. Two isozymes were isolated from rat liver by using ion exchange chromatography. These two isozymes were designated as nPTK-I and nPTK-II, and were found to be minor and major components of total nPTK respectively. These proved to be two different enzymes due to their different characteristics, such as molecular weights, electrical charges and kinetics. The specific activity nPTK-I and its percentages in total nPTK significantly increased in preneoplastic stage (week 10) and slightly elevated further in neoplastic stage (week 18). The specific activity of the nPTK-II moderately increased at week 10, but compared to its value at week 10, it decrease at week 18, resulted in gradual decrease of its percentage in total nPTK. These results indicate that rat hepatic nPTK-I and nTPK-II may be related to neoplastic and preneoplastic stages of rat liver respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01192209
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  • 7
    Electronic Resource
    Electronic Resource
    Changes in N-acetylglucosaminyltransferase III, IV and V in renal cell carcinoma (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 296-299 
    Details
    ISSN: 1432-1335
    Keywords: Key words Renal cell carcinoma ; N-acetylglucosaminyl-transferase ; γ-Glutamyltranspeptidase ; Glycan ; ConA affinity chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The activities of N-acetylglucosaminyltransferase (GnT) III, IV and V were determined in 10 cases of renal cell carcinoma (RCC) and compared with the normal kidney cortex (NKC) regions of the same kidney resected from RCC patients. It was found that the GnT III and GnT IV activities decreased consistently in all samples of RCC, while GnT V activity increased, decreased or did not change in different samples. The mean levels of GnT III and GnT IV activities in RCC were found to be very significantly lower than those of NKC on statistical analysis, but the mean value of GnT V activity was almost identical in RCC and NKC. The decrease in GnT activities in RCC were compatible with the decrease in bisecting N-acetylglucosamine (GlcNAc) and antennary number of complex-type N-glycans in γ-glutamyltranspeptidase (γ-GT) partially purified from RCCs as studied with concanavalin A (ConA) affinity column chromatography, which showed a decrease of unbound fraction and increase of bound fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01208642
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  • 8
    Electronic Resource
    Electronic Resource
    Thermal Decomposition Kinetics of the Zn(II) Complex with Norfloxacin in Static Air Atmosphere (1999)
    Springer
    Journal of thermal analysis and calorimetry 58 (1999), S. 269-278 
    Details
    ISSN: 1572-8943
    Keywords: non-isothermal kinetics ; norfloxacin ; thermal decomposition ; Zn(II) complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The thermal decomposition of Zn[NFA]2⋅5H2O (NFA=C16H18FN3O3, norfloxacin) and its kinetics were studied under non-isothermal conditions in air by TG-DTG and DTA methods. The intermediate and residue for each decomposition were identified from the TG curve. The non-isothermal kinetic data were analyzed by means of the Achar method and the Madhusudanan-Krishnan-Ninan (MKN) method. The possible reaction mechanisms were investigated by comparing the kinetic parameters. The kinetic equation for the second stage can be expressed as dα/dt=Aexp(−E/RT)(1−α).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010142928608
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