Electronic Resource
Copenhagen
:
International Union of Crystallography (IUCr)
Acta crystallographica
55 (1999), S. 988-993
ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
A method is presented for producing a seed-stock mixture for macromolecular crystallization. A PTFE bead and microcentrifuge tube act as mortar and pestle for pulverizing seed crystals of macromolecules. Energy for the bead's motion is supplied by a vortex mixer or an ultrasonic bath. The crushed crystal is serially diluted to prepare a seed-stock mixture of the desired concentration for crystallization. Crystals produced using both hanging-drop vapor diffusion and a capillary microbatch method show expected dilution behavior. This technique of producing seed stock is compared with traditional means and advantages over the standard protocol are demonstrated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444999002085
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