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Portable ocular laser Doppler red blood cell velocimeter (1997)

Logean, E. ; Geiser, M. H. ; Petrig, B. L. ; [et al.]

[S.l.] : American Institute of Physics (AIP)

Review of Scientific Instruments 68 (1997), S. 2878-2882

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ISSN: 1089-7623

Source: AIP Digital Archive

Topics: Physics , Electrical Engineering, Measurement and Control Technology

Notes: A new hand-held bidirectional ocular laser Doppler system for measurement of red blood cell (RBC) velocity in retinal vessels and blood flow in the optic nerve head tissue and in the choriocapillaris is described. Laser power at the cornea is below the maximum permissible level. The instrument is based on the well-established laser Doppler velocimetry/flowmetry techniques. The optical systems for the delivery of the probing laser beam to the eye fundus and bidirectional detection of the light scattered by the RBCs have been implemented in a portable Kowa Genesis fundus camera. In vitro experiments demonstrate the performance of the instrument and in vivo measurements illustrate the feasibility of retinal blood velocity and choriocapillaris blood flow measurements in awake volunteers. © 1997 American Institute of Physics.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1063/1.1148211

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Bioassay for hamster macrophage chemotaxis: application to study particle-lung interactions (1998)

Geiser, M. ; Schüpbach, R. ; Waber, U. ; [et al.]

Springer

Cellular and molecular life sciences 54 (1998), S. 179-185

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ISSN: 1420-9071

Keywords: Key words. Bioassay; chemotaxis; complement; hamster; lung; macrophages; polystyrene particles.

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract. Attraction of lung macrophages to particle deposition sites has been demonstrated in different animal species. We reported a threefold increase of the number of macrophages to occur within 40 min after polystyrene particle deposition in hamster airways [Geiser et al. (1994) Am. J. Respir. Cell Mol. Biol. 160: 594–603]. Complement-derived chemotactic activity is one of the mechanisms postulated for macrophage recruitment. It was the aim of this study to test whether complement-derived chemotactic activity is involved in the rapid recruitment of macrophages to the site of deposited polystyrene particles in hamster airways. We first developed an in vitro cell migration assay for hamster macrophages to assess complement-derived chemotaxis. Second, the bronchoalveolar lavage fluids (BALF) of four hamsters that had inhaled aerosols of polystyrene microspheres were tested for chemotactic activity by this bioassay and compared with BALF of four sham-exposed hamsters. Chemotactic response of macrophages was found toward complement-activated hamster serum, whereas macrophage migration was not increased toward BALF of particle and sham-exposed hamsters. In contrast, macrophage migration to BALF of both groups was reduced by 1.6-fold. Thus, the stimulus for macrophage recruitment to the site of deposited polystyrene particles in hamster airways could not be demonstrated using this bioassay.