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  • 1995-1999  (2)
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Material
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Year
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  • 1
    Electronic Resource
    Electronic Resource
    A quantitative immunocytochemical analysis of total surface area of blood-brain barrier in developing rat brain (1996)
    Springer
    Comparative clinical pathology 6 (1996), S. 214-220 
    Details
    ISSN: 1433-2981
    Keywords: Rat ; Blood-brain barrier ; Development ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A mouse monoclonal antibody which specifically reacts with putative blood-brain barrier (BBB) competent endothelial cells of rat cerebral capillaries was used to identify barrier competent cells in the central nervous system (CNS). The development of the cerebral capillaries and the BBB was examined and quantified, from day 6 to day 40 postpartum, using immunocytochemical and unbiased stereological techniques. There was a progressive increase in capillary formation postnatally, with collateral branching observed with progressive age. BBB development was confined to individual endothelial cells located at the periphery of the cortex until day 10 postpartum. Antibody binding progressively increased postnatally, contributing 30% of the total capillary surface area by day 20. There was a rapid elevation of reactivity from day 20 to day 40, with a mean of 83% by day 40. The BBB constitutes minimal amounts of brain vascular capillaries before day 10 of life in the rat. There is a slower increase in BBB than in total capillaries between days 10 and 20. There is a reversal of this trend between days 20 and 40.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378113
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The central nervous system ofGrillotia erinaceus (Cestoda: Trypanorhyncha) as revealed by immunocytochemistry and neural tracing (1995)
    Springer
    Parasitology research 81 (1995), S. 152-157 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Immunofluorescent labeling and neural tracing techniques were employed in conjunction with confocal scanning laser microscopy to study the intact neuroanatomy of the central nervous system of the trypanorhynch tapewormGrillotia erinaceus Immunocytochemical labeling for the general nerve fibre marker PGP 9.5 showed a pattern of extensive labeling that paralleled findings obtained with the neural tracer DiI. In constrast, immunocytochemical labeling for 5-hydroxytryptamine (5-HT) was localised to cell bodies lying on the periphery of the ganglion, with fine immunoreactive fibres radiating out towards the bothridia. Following the retrograde transport of the fluorescent molecule DiI through axotomised nerve cords, it successfully labeled both the cerebral ganglion and associated nerve fibres within the scolex. The cerebral ganglion was shown to give rise to posterior nerve cords, an array of radial fibres that pass out to the bothridia, and to contain a centrally disposed group of cell bodies thought to be involved in efferent functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931622
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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