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  • 1
    Electronic Resource
    Electronic Resource
    Ultraviolet-B-induced stress and changes in gene expression in Arabidopsis thaliana: role of signalling pathways controlled by jasmonic acid, ethylene and reactive oxygen species (1999)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 22 (1999), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Treatment with supplementary UV-B resulted in decreases in transcripts of the photosynthetic genes Lhcb and psbA and concomitant increase in transcripts of two pathogen-related genes, PR-1 and PDF1·2, in Arabidopsis thaliana. UV-B exposure caused increases in jasmonic acid (JA) levels and ethylene production. UV-B treatment of jar1 and etr1-1 mutants, which are insensitive to JA and ethylene, respectively, showed that the increase in PR-1 transcripts was dependent on ethylene and PDF1·2 transcripts on both JA and ethylene. In contrast, the down-regulation of photosynthetic transcripts was independent of both compounds. Previous studies have indicated a role for reactive oxygen species (ROS) in the UV-B-induced down-regulation of the photosynthetic genes and up-regulation of PR-1 genes. Here we have shown that ROS are also required for the UV-B-induced up-regulation of PDF1·2 genes. The results indicate that the effects of UV-B on the three sets of genes are mediated through three distinct signal transduction pathways which are similar, but not identical, to pathways initiated in response to pathogen infection. In addition, the increased sensitivity of both jar1 and etr1-1 mutants to UV-B radiation, as compared with wild-type plants, indicated that intact JA and ethylene signal pathways are required for defence against UV-B damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.1999.00499.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ultraviolet-B-induced DNA lesions and their removal in wheat (Triticum aestivum L.) leaves (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 19 (1996), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Monoclonal antibodies were used in an enzyme-linked immunosorbent assay (ELISA) to detect the induction and removal of cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts in DNA isolated from ultraviolet B (UV-B)-exposed primary wheat (Triticum aestivum L. cv. Chinese Spring) leaf tissue. The accumulation of lesions in the primary leaves of 6-d-old wheat seedlings was followed during the exposure of the leaf to an approximate dose of 3.6×10−1 W m−2 UV-B (Caldwell weighting). Significant increases in the levels of both CPDs and (6-4) photoproducts were detected in wheat leaves exposed to UV-B in the absence of other light However, only an increase in (6-4) photoproduct levels could be measured in wheat leaves exposed to the same UV-B source in the presence of supplemental white light. The removal of CPD antibody binding sites in the DNA after irradiation was rapid under conditions of high light intensity in contrast to the removal of (6-4) photoproduct antibody binding sites, which was significantly slower. The removal of CPDs appeared to be light dependent, this rate of removal decreasing with decreasing light fluences. The removal of (6-4) photoproducts also appeared light dependent, but to a lesser extent than the removal of CPDs, under the conditions studied here. Gene expression in the primary wheat leaf was measured and showed an up-regulation of chalcone synthase expression and a reduction in expression of chlorophyll a/b-binding protein (cab) in response to supplementary UV-B. No effect was seen on the expression of the other photosynthetic genes studied (the genes coding for the enzymes sedoheptu-lose 1,7-bisphosphatase and fructose 1,6-bisphosphatase). Measurement of the levels of DNA lesions in this same tissue showed that the observed changes in gene expression accompanied the appearance of UV-B induced lesions in the form of (6-4) photoproducts in the wheat leaf genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1996.tb00238.x
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  • 3
    Electronic Resource
    Electronic Resource
    Ultraviolet-B-induced responses in Arabidopsis thaliana: role of salicylic acid and reactive oxygen species in the regulation of transcripts encoding photosynthetic and acidic pathogenesis-related proteins (1998)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 21 (1998), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Supplementary UV-B was shown to lead to a decrease in transcripts encoding the photosynthetic genes Lhcb and psbA and a concomitant increase in transcripts encoding three acid-type pathogenesis-related proteins, PR-1, PR-2 and PR-5, in Arabidopsis thaliana. UV-B radiation has been reported to lead to the generation of reactive oxygen species (ROS). Here we report that ROS are required for UV-B-induced down-regulation of the photosynthetic genes and up-regulation of PR genes, as the addition of antioxidants before UV-B treatment resulted in a marked reduction in the effect of UV-B on both sets of genes. Rises in ROS are frequently accompanied by increases in salicylic acid (SA) accumulation. UV-B treatment of transgenic NahG Arabidopsis plants, which are unable to accumulate SA, showed that the increase in PR transcripts, but not the decrease in photosynthetic transcripts, was dependent on the increase in SA. In addition, a 3 d exposure to UV-B radiation resulted in a 7-fold increase in SA levels. Oxidant treatment of NahG plants indicated that ROS could not up-regulate PR genes in the absence of SA accumulation; however, the down-regulation of photosynthetic transcripts was unchanged from that in wild-type plants. The results indicate that the effects of UV-B on the two sets of genes are mediated through two distinct signal tranduction pathways. One pathway is ROS-dependent but SA-independent and mediates the down-regulation of photosynthetic genes. The other is SA- and ROS-dependent and mediates the up-regulation of the acidic-type PR genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.1998.00325.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ultraviolet-B effects on transcript levels for photosynthetic genes are not mediated through carbohydrate metabolism (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 20 (1997), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The relationship between UV-B-induced changes in gene expression and carbohydrate levels in pea seedlings has been investigated. The effect of supplementary UV-B radiation on the transcript abundance for two photosynthetic genes, photosynthesis, respiration and the levels of carbohydrates was determined in fully expanded leaves of 17-d-old pea seedlings under high (HL: 350 μmol m−2 s−1) and low (LL: 150 μmol m−2 s1) light. Supplementary UV-B caused down regulation of the photosynthetic genes in green leaves under LL and to a lesser extent under HL. In contrast to previous studies, UV-B radiation resulted in a decrease in glucose levels rather than an increase under LL. Sucrose and starch levels were not affected until longer exposure. Effects of UV-B on carbohydrate levels were, however, minimal under HL. The effects on transcript levels were most marked under LL and therefore could not be attributed to elevated carbohydrate levels. Comparison of UV-B effects on carbohydrates in source (leaf) and sink (green buds) organs indicated that changes in carbohydrates in response to UV-B are probably indirect and arise from effects of UV-B on photosynthesis in source organs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.1997.d01-39.x
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  • 5
    Electronic Resource
    Electronic Resource
    Fighting as a Function of Personality and Neuropsychological Measures (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 794 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb32558.x
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  • 6
    Electronic Resource
    Electronic Resource
    Co-Evolution in the Successful Learning of Backgammon Strategy (1998)
    Springer
    Machine learning 32 (1998), S. 225-240 
    Details
    ISSN: 0885-6125
    Keywords: coevolution ; backgammon ; reinforcement ; temporal difference learning ; self-learning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Following Tesauro's work on TD-Gammon, we used a 4,000 parameter feedforward neural network to develop a competitive backgammon evaluation function. Play proceeds by a roll of the dice, application of the network to all legal moves, and selection of the position with the highest evaluation. However, no backpropagation, reinforcement or temporal difference learning methods were employed. Instead we apply simple hillclimbing in a relative fitness environment. We start with an initial champion of all zero weights and proceed simply by playing the current champion network against a slightly mutated challenger and changing weights if the challenger wins. Surprisingly, this worked rather well. We investigate how the peculiar dynamics of this domain enabled a previously discarded weak method to succeed, by preventing suboptimal equilibria in a “meta-game” of self-learning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007417214905
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  • 7
    Electronic Resource
    Electronic Resource
    Differential gene expression in CD3e- and RAG1-deficient thymuses: definition of a set of genes potentially involved in thymocyte maturation (1999)
    Springer
    Immunogenetics 50 (1999), S. 255-270 
    Details
    ISSN: 1432-1211
    Keywords: Key words Thymus ; T lymphocytes ; Gene regulation ; Chemokines ; Thymic stroma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A set of 3000 mouse thymus cDNAs was analyzed by extensive measurement of expression using complex-probe hybridization of DNA arrays ("quantitative differential screening"). The complex probes were initially prepared using total thymus RNA isolated from C57BL/6 wild-type (WT), CD3e- and RAG1-deficient mice. Over 100 clones displaying over- or under-expression by at least a factor of two between WT and knockout (KO) thymuses were further analyzed by measuring hybridization signatures with probes from a wide range of KO thymuses, cell types, organs, and embryonic thymuses. A restricted set of clones was selected by virtue of their expression spectra (modulation in KO thymuses and thymocytes, lymphoid cell specificity, and differential expression during embryonic thymus development), sequenced at one extremity, and compared to sequences in databases. Clones corresponding to previously identified genes (e.g., Tcrβ, Tcf1 or CD25) showed expression patterns that were consistent with existing data. Ten distinct clones corresponding to new genes were subjected to further study: Northern blot hybridization, in situ hybridization on thymus sections, and partial or complete mRNA sequence determination. Among these genes, we report a new serine peptidase highly expressed in cortical epithelial cells that we have named thymus-specific serine peptidase (TSSP), and an acidic protein expressed in thymocytes and of unknown function that we have named thymus-expressed acidic protein (TEAP). This approach identifies new molecules likely to be involved in thymocyte differentiation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050601
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  • 8
    Electronic Resource
    Electronic Resource
    Modulation of mRNA levels in the presence of thymocytes and genome mapping for a set of genes expressed in mouse thymic epithelial cells (1997)
    Springer
    Immunogenetics 46 (1997), S. 142-151 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Modulation of gene expression in mouse thymic epithelium upon culture in the presence of thymocytes (coculture) was studied by comparison of hybridization signatures on a set of nearly 5000 mouse thymus cDNA clones. Forty-nine differentially expressed clones (usually down-regulated in coculture) were characterized by tag sequencing. Many of them corresponded to entities that had not been described previously in the mouse, and were further characterized by genome mapping. This set of genes appears to be involved in growth regulation and differentiation within the thymus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050253
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