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  • 1
    Electronic Resource
    Electronic Resource
    Regulatory Mechanism of Pepsinogen Gene Expression in Monolayer Cultured Guinea Pig Gastric Chief Cells (1998)
    Springer
    Digestive diseases and sciences 43 (1998), S. 2744-2749 
    Details
    ISSN: 1573-2568
    Keywords: PEPSINOGEN mRNA ; cAMP ; INTRACELLULAR CALCIUM ; PROTEIN KINASE C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have investigated the effects of dibutyrylcAMP, forskolin, carbamylcholine chloride (carbachol),ionomycin, and 12-O-tetradecanoylphorbol-13-acetate(TPA) on the expression of guinea pig pepsinogen mRNA in monolayer cultured gastric chief cells.After exposure of the cells to each of these compoundsfor 4 to 24 hr, and at 48 hr after primary culture,total cellular RNA was isolated using acidguanidium-phenol-chloroform and then was reverse transcribed to cDNA.Obtained cDNA was amplified by polymerase chain reaction(PCR) using primers detecting guinea pig pepsinogen mRNAand human β-actin mRNA as an internal standard. The PCR products were separated and quantifiedusing capillary electrophoresis. Dibutyryl cAMP andforskolin significantly increased pepsinogen mRNA, butcarbachol, ionomycin, and TPA failed to increase that. These findings suggested that pepsinogengene expression was up-regulated by intracellular cAMP,but not by intracellular calcium or protein kinase C inguinea pig chief cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026623932350
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  • 2
    Electronic Resource
    Electronic Resource
    Mitogenic Properties of Human Gastric Juice (1997)
    Springer
    Digestive diseases and sciences 42 (1997), S. 1747-1754 
    Details
    ISSN: 1573-2568
    Keywords: GASTRIC JUICE ; GROWTH FACTOR ; MITOGENIC ACTIVITY ; EPIDERMAL GROWTH FACTOR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was performed to define thebiologically active growth modulators in human gastricjuice. Mitogenic activity was evaluated by theincorporation of [3H]thymidine into 3T3fibroblasts. A negative correlation was observed between pH andmitogenic activity in gastric juice (r = –0.45, P〈 0.01). The concentrations of epidermal growthfactor (EGF), transforming growth factor-α and-β1 (TGF-α and -β1), platelet-derived growth factor 1(PDGF), and basic fibroblast growth factor (bFGF) ingastric juice did not explain these changes in mitogenicactivity. Gel filtration identified growth-stimulatingactivity due to small molecule mitogens (less than 13kDa), and growth inhibitory activity only in neutralsamples due to a macromolecular substance (larger than240 kDa) susceptible to trypsin digestion and heat and acid treatments. We conclude thatacidity-dependent changes in mitogenic activity observedin this study are due to appearance of acid-unstable,high-molecular-weight, growth-inhibitorysubstance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018877918894
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Epidermal growth factor enhances repair of rat intestinal mucosa damaged by oral administration of methotrexate (1995)
    Springer
    Journal of gastroenterology 30 (1995), S. 169-176 
    Details
    ISSN: 1435-5922
    Keywords: ornithine decarboxylase ; α-difluoromethylornithine ; diamine oxidase ; chemotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To examine the trophic effect of epidermal growth factor on the rat small intestine, we measured diamine oxidase and ornithine decarboxylase activities in intestinal mucosa injured by methotrexate. Methotreaate was infused orally via a gastric tube at a dose of 10 mg/kg per day on 3 successive days (days 1–3). Epidermal growth factor was injected intraperitoneally at a dose of 40 μg/kg per day on 4 successive days following methotrexate infusion (days 4–7). Methotrexate caused a marked decrease in diamine oxidase activity; this decrease returned to a normal level on day 13 in controls. In rats injected with epidermal growth factor, diamine oxidase activity began to recover earlier than in the controls, and returned to a normal level on day 11. Epidermal growth factor enhanced the increase of ornithine decarboxylase activity in mucosa injured by methotrexate. When the increase of ornithine decarboxylase activity was suppressed by α-difluoromethylornithine, epidermal growth factor failed to facilitate the repair of intestinal mucosa. These results indicate that epidermal growth factor enhances intestinal repair following methotrexate infusion, and that this effect is mediated, at least in part, by ornithine decarboxylase. It is proposed that epidermal growth factor can be used clinically as a means to enhance mucosal repair of the intestine after chemotherapy with methotrexate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02348661
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  • 4
    Electronic Resource
    Electronic Resource
    Lymphoma arising in mucosa-associated lymphoid tissue of the duodenal bulb (1998)
    Springer
    Journal of gastroenterology 33 (1998), S. 97-101 
    Details
    ISSN: 1435-5922
    Keywords: Key words: mucosa-associated lymphoid tissue ; lymphoma ; duodenum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: We report a case of low-grade B-cell lymphoma of the duodenal bulb arising from mucosa-associated lymphoid tissue. A barium swallow and an endoscopic examination showed multiple elevated, irregularly contoured lesions limited to the duodenal bulb. Endoscopic biopsy specimens were highly suggestive of non-Hodgkin's lymphoma. The resected specimen showed a gyriform mucosal elevation measuring 3 × 2 cm in extent, with multiple small poly-poid elevations scattered around it. Histologically, the small lymphocytes constituting the tumor infiltrated the duodenal mucosa and submucosa. The neoplastic centrocyte-like cells tended to grow around reactive lymphoid follicles and to invade epithelial structures, forming characteristic lymphoepithelial lesions. Monoclonal proliferation of the lymphoid tissue was demonstrated by the polymerase chain reaction method. The histologic appearance and the demonstration of monoclonality fulfilled the criteria for malignant lymphoma arising from mucosa-associated lymphoid tissue, which is extremely rare in the duodenum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005350050051
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    Paper (German National Licenses)
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