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  • 1995-1999  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Preparation of stearoyl lactic acid ester catalyzed by lipases from Rhizomucor miehei and porcine pancreas optimization using response surface methodology (1999)
    Springer
    Applied microbiology and biotechnology 52 (1999), S. 579-584 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The esterification reaction between stearic acid and lactic acid using Rhizomucor miehei lipase and porcine pancreas lipase was optimized for maximum esterification using response surface methodology. The formation of the ester was found to depend on three parameters namely enzyme/substrate ratio, lactic acid (stearic acid) concentration and incubation period. The maximum esterification predicted by theoretical equations for both lipases matched well with the observed experimental values. In the case of R. miehei lipase, stearoyl lactic acid ester formation was found to increase with incubation period and lactic acid (stearic acid) concentrations with maximum esterification of 26.9% at an enzyme/substrate (E/S) ratio of 125 g mol−1. In the case of porcine pancreas lipase, esterification showed a steady increase with increase in incubation period and lactic acid (stearic acid) concentration independent of the E/S ratios employed. In the case of PPL, a maximum esterification of 18.9% was observed at an E/S ratio of 25 g mol−1 at a lactic acid (stearic acid) concentration of 0.09 M after an incubation period of 72 h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051563
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterisation of a starch-hydrolysing enzyme of Aspergillus niger (1999)
    Springer
    Applied microbiology and biotechnology 51 (1999), S. 673-675 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A UV-induced mutant strain of Aspergillus niger (CFTRI-1105-U9) overproduced a starch-hydrolysing enzyme with properties characteristically different from the known amylases of the fungus. The purified enzyme of 4.0 pI had an apparent molecular mass of 125 kDa and it dextrinised starch and then saccharified the dextrins. Patterns of the enzyme activity on starch, resulting in glucose at 60 °C and glucose, maltose and maltodextrins at 70 °C as primary products, suggested significant applications for the enzyme in starch-processing industries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051450
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    An enzyme-linked immunosorbent assay for the estimation of fungal biomass during solid-state fermentation (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 299-302 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An enzyme-linked immunosorbent assay for sensitive, specific and quantitative estimation of fungal biomass during solid-state fermentation is described. Using this method, differential growth rates and colonization of the substrate can be studied. The assay has potential application for the efficient monitoring of solid-state fermentation involving specific fungus, for which available methods are not adequate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051294
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Isomerization of non-insecticidal α-hexachlorocyclohexane (HCH) to insecticidal γ-HCH by Pseudomonas strain Ptm+ (1999)
    Springer
    Applied microbiology and biotechnology 53 (1999), S. 120-126 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Pseudomonas strain Ptm+ grew on α-hexa-chlorocyclohexane (HCH, CAS no. 319846), using it as the sole source of carbon and energy. In a replacement-culture study, with the non-insecticidal α-HCH, γ-HCH (CAS no. 58899) was the first metabolite noticed at 6 h, and transient accumulation of insecticidal γ-HCH occurred for up to 18 h. Although delta- (CAS no. 319868) and beta-isomers (CAS no. 319857) were also detected, their concentrations were very low. By 18 h of incubation, about 23% of the α-HCH added was transformed into the gamma-isomer. Subsequently, the concentration of γ-HCH in the medium fell. Thin-layer chromatography, gas chromatography, gas chromatography-mass spectrometry and mosquito-larval bioassay analyses confirmed the formation of γ-HCH. This was associated with the formation of three more metabolites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051624
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Screening and selection of media components for lactic acid production using Plackett–Burman design (1998)
    Springer
    Bioprocess engineering 19 (1998), S. 61-65 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Plackett–Burman design was used to efficiently select important media components influencing lactic acid production in a two step screening procedure. A total of 36 screening experiments were conducted for studying the effect of various media components such as carbon and nitrogen (simple and complex) sources, minerals/buffering agents and a specific inducer for the production of lactic acid by Lactobacillus plantarum NCIM 2084. The eleven ingredients chosen after the first screening experiments were further screened by a Plackett-Burman design consisting of 12 experiments. Liquefied starch, wheat bran extract, ammonium nitrate, manganese sulphate and sodium acetate were chosen as promising ingredients for further optimisation studies. The highest yield of 41.9 g/l of lactic acid was obtained at the end of 24 hours of fermentation which corresponded to 90% conversion, on the basis of sugar supplied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00009003
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    On-line monitoring of ethanol: in relation to the rate of carbon dioxide evolved (1999)
    Springer
    Biotechnology techniques 13 (1999), S. 363-364 
    Details
    ISSN: 1573-6784
    Keywords: continuous fermentation ; ethanol and CO2 evolution rate ; on line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple method for rapid on-line measurement of ethanol, produced in a continuous bioreactor, in a steady state, is described. Soap film meter measures the rate of CO2 evolved. This value is equated to the equimolar production of ethanol. A mean 3.3% error was observed, as compared to the gas chromatography method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008905414904
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    Paper (German National Licenses)
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