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  • 1
    Electronic Resource
    Electronic Resource
    DNA-Binding Studies of a Bifunctional Platinum Complex That Is a Luminescent Intercalator (1995)
    s.l. : American Chemical Society
    Inorganic chemistry 34 (1995), S. 4484-4489 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00121a029
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Luminescence Quenching of Copper(II) Porphyrins with Lewis Bases (1995)
    s.l. : American Chemical Society
    Inorganic chemistry 34 (1995), S. 2015-2018 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00112a011
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    On the lability and functional significance of the type 1 copper pool in ceruloplasmin (1999)
    Springer
    JBIC 4 (1999), S. 441-446 
    Details
    ISSN: 1432-1327
    Keywords: Key words Ceruloplasmin ; Copper transport ; Ferrooxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract  The possibility that ceruloplasmin (CP) functions as a copper transferase has fueled a continuing interest in studies of the copper release process. The principal goal of the current investigation has been to identify the most labile copper centers in sheep protein. In fact, subjecting the enzyme to a slow flux of cyanide at pH 5.2 under nitrogen in the presence of ascorbate and a phenanthroline ligand produces partially demetalated forms of the protein. By standard chromatographic techniques it is possible to isolate protein with a Cu/CP ratio of ∼4 or ∼5 as opposed to the native protein which has Cu/CP=5.8. In contrast to other blue oxidases, analysis suggests that CP preferentially loses its type 1 coppers under these conditions. Thus, the spectroscopic signals from the type 1 centers exhibit a loss of intensity while the EPR signal of the type 2 copper becomes stronger. Furthermore, the Cu/CP≈4 and Cu/CP≈5 components retain about 50% of the activity of the native protein, consistent with an intact type 2/type 3 cluster. All three type 1 copper sites appear to suffer copper loss. Reconstitution with a copper(I) reagent restores the spectroscopic properties of the native protein and 90% of the original activity. The results suggest a possible functional significance for the presence of three type 1 coppers in CP. By employing a pool of redox-active but relatively labile type 1 copper centers, the enzyme can serve as a copper donor, if necessary, without completely sacrificing its oxidase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007750050330
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of genetic variation in Bradyrhizobium japonicum USDA 110 variants using DNA fingerprints generated with GC rich arbitrary PCR primers (1996)
    Springer
    Plant and soil 186 (1996), S. 81-85 
    Details
    ISSN: 1573-5036
    Keywords: Bradyrhizobium japonicum colony morphology variants ; DNA fingerprints ; GC rich arbitrary primers RAPD-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bradyrhizobium japonicum USDA 110 has been shown to contain several genetically similar naturally occurring colony morphology variants. These variants differ in symbiotic nitrogen fixation ability and in the utilization of various carbon substrates. They have been shown to share extensive DNA homology and appear to be derived from a common ancestor. Despite these similarities certain B. japonicum USDA 110 variants have been shown to be devoid of symbiotic nitrogen fixation. One of these variants (L2-110), however, was recently shown to possess significant levels of explanta nitrogen fixation and to synthesize functional dinitrogenase enzyme within bacteroids. In an effort to identify genetic markers which could explain differences in symbiotic nitrogen fixation between B. japonicum variants, DNA fingerprints were generated by PCR using arbitrary primers. Two of these primers with GC rich sequences were able to differentiate between B. japonicum USDA 110 variants I-110, L2-110, and MN-110. Unique markers have now been identified which could be examined further to determine if they explain the differences in symbiotic nitrogen fixation between USDA 110 variants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035059
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  • 5
    Electronic Resource
    Electronic Resource
    Detection and Transcription of Toxin DNA in a Nontoxigenic Strain of Clostridium difficile (1999)
    Springer
    Current microbiology 38 (1999), S. 324-328 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Genomic DNA from three Clostridium difficile strains was analyzed by PCR for DNA sequences encoding toxin A (tcdA) and toxin B (tcdB). Toxigenic control strain VPI 10463 possessed tcdA, tcdB, and an open reading frame (tcdE) between these two genes, whereas nontoxigenic control strain 85 lacked each of these genetic determinants. However, strain M90, also a nontoxigenic strain, was found to possess tcdA, tcdB, and tcdE. Normally the presence of toxin genes is associated with toxigenicity. Analysis of tcdA and tcdB mRNA revealed toxin gene transcription in strains VPI 10463, 23 (a mildly toxigenic strain), and M90, but not in strain 85. However, for strain M90, tcdA and tcdB mRNA was at the lower limit of detection, whereas mRNAs encoding tcdA and tcdB were easily detected in strains VPI 10463 and 23. Low levels of toxin gene transcription is the probable cause of M90's lack of toxigenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006811
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic Variation in Two Cultures of Bradyrhizobium japonicum 110 Differing in Their Ability to Impart Drought Tolerance to Soybean (1997)
    Springer
    Current microbiology 35 (1997), S. 363-366 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The polymerase chain reaction with arbitrary primers (RAPD) discriminated between two separately maintained cultures of Bradyrhizobium japonicum USDA 110 differing in symbiotic performance under drought conditions. Since strain 110 is used in inoculum production, the use of RAPD to monitor inoculum cultures could help to preserve their genetic composition and prevent the loss of important symbiotic properties. The use of RAPD could also be extended to other B. japonicum strains currently used in inoculum production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002849900269
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    Paper (German National Licenses)
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