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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @Muslim world 89 (1999), S. 0 
    ISSN: 1478-1913
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Theology and Religious Studies
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 141 (1999), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 800 (1996), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 154 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We have previously produced and characterized four monoclonal antibodies to the Brucella DnaK protein which were derived from mice infected with B. melitensis or immunized with the B. melitensis cell wall fraction. By use of a recombinant DNA technique, we have localized a linear epitope, recognized by two of these monoclonal antibodies (V78/07B01/G11 and V78/09D04/D08), in the last 21 amino acids of the C-terminal region of the Brucella DnaK protein. The C-terminal region has been reported to be the most variable region among DnaK proteins. The two other monoclonal antibodies (A53/09G03/D02 and A53/01C10/A10) failed to react with the recombinant clones and might recognize discontinuous epitopes of the Brucella DnaK protein. The four monoclonal antibodies reacted with all recognized Brucella species and biovars in immunoblotting after SDS-PAGE. Monoclonal antibodies V78/07B01/G11 and V78/09D04/D08 did not react with reported cross-reacting bacteria nor with bacteria of the α-2 subdivision of the class Proteobacteria for which a close genetic relationship with Brucella spp. has been reported. However, monoclonal antibodies A53/09G03/D02 and A53/01C10/A10 reacted with Phyllobacterium rubiacearum and/or Ochrobactrum anthropi, both bacteria of the α-2 subdivision of the class Proteobacteria. The Brucella genus DnaK specific epitopes could be of importance for diagnostic purposes.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract We have previously identified a Brucella melitensis 28 kDa cytosoluble protein (CP28) which was highly immunogenic in infected sheep and which in addition made possible the serological differentiation between infected and B. melitensis Rev.l vaccinated sheep. Monoclonal antibodies against CP28 were used to screen a B. melitensis 16M genomic library and to clone the corresponding gene. DNA sequencing of the gene encoding CP28 of B. melitensis 16M revealed that it was nearly identical to that of the recently published bp26 gene of Brucella abortus vaccine strain S19 coding for a periplasmic protein. The differences between the B. melitensis 16M gene and that of B. abortus S19 consisted of single nucleotide substitutions, one or two codon deletions, one codon addition, and most importantly a 21-bp deletion. The corresponding region of B. abortus S19 contains two 10-bp direct repeats which could have been involved in the genesis of the deletion. Expression of the B. melitensis 16M bp26 gene in Escherichia coli studied by the use of the monoclonal antibodies showed the same characteristics as reported for the B. abortus S19 bp26 gene, i.e. the presence of a higher molecular mass preprotein and a lower molecular mass band which probably corresponds to the mature protein exported to the periplasm. Immunoblotting performed with sera from either naturally infected or B. melitensis H38 experimentally infected sheep confirmed the importance of the B. melitensis CP28/BP26 protein as diagnostic antigen.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric surgery international 10 (1995), S. 148-151 
    ISSN: 1437-9813
    Keywords: Blunt chest trauma ; Tracheobronchial laceration ; Mediastinal emphysema ; Pneumoperitoneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 6-year-old boy sustained a crush injury to the upper chest that resulted in a linear laceration of the membranous part of the lower trachea and right main stem bronchus. He presented with extensive subcutaneous emphysema, pneumomediastinum, and pneumoperitoneum. The diagnosis was indicated by a CT scan of the chest and confirmed by bronchoscopy. The trachea was intubated in the operating room after rigid bronchoscopy. Intraoperative leakage of gas from the tracheobronchial laceration was minimized by single left-lung manual ventilation and application of moist packs to the site of tracheal injury. Surgical repair was achieved with interrupted sutures of non-absorbable material; a pedicle pleural flap was used to buttress the suture line. The patient was discharged in good condition after 1 week. Complete healing of the anastomosis was attested at bronchoscopy 2 months postoperatively. The rarity of tracheobronchial lacerations in childhood is noted on review of the literature. Emphasis is placed on early recognition and expeditious management of tracheobronchial lacerations. Rarely, these lacerations have been reported to heal spontaneously.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Gütegeschalteter Rubinlaser ; Tätowierungen ; Lentigines ; Key words Q-switched ruby laser ; Tattoos ; Lentigines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The Q-switched ruby laser (QSRL) with its wavelength of 694 nm and a pulse duration of around 40 nsec is an effective modality for the removal of tattoos and cutaneous pigmented lesions. Based on the principle of selective photothermolysis, selective damage to cutaneous pigment or pigmented cells is possible, allowing the scar-free elimination of endogenous or exogenous pigment in the skin. Main indications for the treatment with the QSRL are tattoos (amateur, professional, accidental, or cosmetic) and lentigines but the QSRL can also be used for lightening or even removing other pigmented lesions such as nevus spilus or café au lait macules. Furthermore, pigmented lesions of mucous membranes can be removed easily. Since treatment results in postinflammatory hyperpigmentation, melasma, and Becker’ nevus have proven to be inconsistent, the QSRL cannot be routinely recommended for these lesions. Melanocytic lesions are generally not treated, with the exception of nevus of Ota and nevus of Ito where there exacts a lack of therapeutic alternatives. Non-pigmented cells, which exist in nearly all melanocytic lesions, do not absorb the light of the QSRL and, therefore, do not react to this particular treatment. No information is available on the risk of partially damaged cells to become malignant after QSRL treatment. The QSRL is an excellent therapy for the removal of endogenous and exogenous pigment because of both the excellent treatment results and the lack of side effects, which are limited to transient hypo- and hyperpigmentation. The QSRL has accurred a wide range of applications within the field of dermatology.
    Notes: Zusammenfassung Der gütegeschaltete Rubinlaser mit einer Wellenlänge von 694 nm und einer Impulszeit im Nanosekundenbereich stellt eine effektive Behandlungsmöglichkeit für Tätowierungen und kutane pigmentierte Hautveränderungen dar. Aufgrund der selektiven Photothermolyse, dem Wirkungsprinzip des Rubinlasers, ist eine selektive Schädigung der Pigmentpartikel bzw. der pigmenttragenden Zellen in der Dermis möglich. Dadurch ist eine narbenfreie Entfernung von endogenem und exogenem Pigment möglich. Wichtige und häufige Indikationen für die Anwendung des gütegeschalteten Rubinlasers sind Tätowierungen (Amateur- und Profitätowierungen, Schmutztätowierungen, Permanent-Make-Up) und Lentigines solares. Der Rubinlaser eignet sich auch zur Behandlung weiterer pigmentierter Hautveränderungen wie Naevus spilus oder Cafe-au-lait-Flecken. Auch Veränderungen der Mundschleimhaut und der Lippen (Lentigines) können mit dem Rubinlaser problemlos entfernt werden. Da die Behandlung von postinflammatorischen Hyperpigmentierungen, Chloasma/Melasma und Becker-Nävi unterschiedliche Ergebnisse aufweist, ist der gütegeschaltete Rubinlaser für diese Indikationen nur eingeschränkt zu empfehlen. Prinzipiell ist von der Behandlung melanozytärer Hautveränderungen mit dem Rubinlaser abzuraten. Ausnahmen bilden der Nävus Ota sowie der Nävus Ito, da hier keine anderen Therapiemodalitäten zur Verfügung stehen. Nichtpigmentierte Zellen, die in fast allen melanozytären Hautveränderungen vorhanden sind, absorbieren das Rubinlaserlicht nicht und sind somit der Behandlung nicht zugänglich. Ob partiell geschädigte Zellen nach Rubinlaserbehandlung möglicherweise ein größeres Entartungsrisiko besitzen, ist nicht bekannt. Bei richtiger Indikationsstellung ist der gütegeschaltete Rubinlaser eine hervorragende Behandlungsmethode zur Entfernung von endogenem und exogenem Pigment der Haut. Aufgrund der Behandlungserfolge und der im Vergleich zu anderen Therapiemöglichkeiten geringen Nebenwirkungen, die sich auf eine transiente Hyper- oder Hypopigmentierung beschränken, hat der Rubinlaser sicher ein festes Anwendungsspektrum in der Dermatotherapie.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Der Hautarzt 49 (1998), S. 192-196 
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Säuglingshämangiome ; Farbstofflaser (FPDL) ; Kryotherapie ; Key words Childhood hemangioma ; Flashlamp-pumped pulsed dye laser (FPDL) ; Cryotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In our comparative study, 40 infants ranging in age from 1 week to six months, were treated for their hemangiomas. Cryosurgery was performed on 20 hemangiomas whereas the others were treated with flash lamp-pumped pulsed dye laser (FPDL). In 72% of initial macular, flat-topped, superficial hemangiomas a predominant (〉75%) or complete regression could be achieved. In 68% of cutaneous-exophytic lesions, both therapeutical modalities showed a sufficient regression (〉50%) or marked growth inhibition after two sessions with further improvement after additional treatment. Cutaneous hemangiomas and the cutaneous part of combined hemangiomas responded well to both therapies whereas subcutaneous hemangiomas were predominantly influenced by cryotherapy. Our data emphasise the efficiency of early treatment of childhood hemangiomas. Since FPDL therapy is easy to perform, less time-consuming and reduces post-therapeutic exsudation, this treatment is preferable in initial macular, flat-topped and superficial lesions while cryotherapy is considered to be superior in lesions with marked elevated and/or subcutaneous parts.
    Notes: Zusammenfassung In einer Vergleichsstudie behandelten wir 40 Säuglinge im Alter zwischen 1 Woche und 6 Monaten. Zwanzig Hämangiome wurden kontaktkryochirurgisch und weitere 20 Hämangiome wurden mittels blitzlampengepumptem, gepulstem Farbstofflaser behandelt. Bei den planen und initialen Hämangiomen ließ sich bei 72% nach einer Behandlung mit beiden Therapieverfahren eine überwiegende (〉75%) bis vollständige Rückbildung erzielen. Bei den kutan-exophytischen Läsionen zeigten sich bei 68% nach der zweiten Behandlung beide Verfahren in der Lage, eine befriedigende Rückbildung (〉50%) bzw. einen markanten Wachstumsstop zu induzieren. Zusätzliche Behandlungszyklen verbesserten diese Ergebnisse nochmals. Die kutanen Anteile der kombinierten kutan-subkutanen Angiome sprachen wie die isolierten, kutan exophytischen Läsionen auf beide Modalitäten an. Deren subkutane Anteile schienen, wenn überhaupt, in erster Linie mit der Kryotherapie beeinflußbar. Unsere Ergebnisse unterstreichen die Effektivität einer Frühtherapie von Säuglingshämangiomen. Bei planen, flächigen und initialen Läsionen geben wir wegen der geringeren posttherapeutischen Exsudation und der schneller durchzuführenden Behandlung dem FPDL den Vorzug. Bei ausgeprägter Exophytie und/oder subkutanem Anteil sehen wir Vorteile beim Kryokontaktverfahren.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1619-7089
    Keywords: Key words: Familial amyloid polyneuropathy ; Metaiodobenzylguanidine ; Heart rate variability ; Autonomic nervous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Familial amyloid polyneuropathy (FAP) is a rare and severe hereditary form of amyloidosis, due to nervous deposits of a genetic variant transthyretin produced by the liver and characterized by both sensorimotor and autonomic neuropathy. Left ventricular systolic dysfunction is rare, but conduction disturbances and sudden deaths can occur. The neurological status of the heart has not been elucidated, and an alteration of the sympathetic nerves may be involved. We studied 17 patients (42±12 years) before liver transplantation by iodine-123 metaiodobenzylguanidine (MIBG) scintigraphy, heart rate variability analysis, coronary angiography, radionuclide ventriculography, rest thallium single-photon emission tomography (SPET) and echocardiography. Coronary arteries, left ventricular systolic function and rest thallium SPET were normal in all patients. Only mild evidence of amyloid infiltration was found at echocardiographic examination. Cardiac MIBG uptake was dramatically decreased in patients compared with age-matched control subjects (heart-to-mediastinum activity ratio at 4 h: 1.36±0.26 versus 1.98±0.35, P〈0.001), while there was no difference in MIBG washout rate. Heart rate variability analysis showed a considerable scatter of values, with high values in four patients despite cardiac sympathetic denervation as assessed by MIBG imaging. The clinical severity of the polyneuropathy correlated with MIBG uptake at 4 h but not with the heart rate variability indices. Cardiac MIBG uptake and the heart rate variability indices did not differ according to the presence or absence of conduction disturbances. Patients with FAP have sympathetic cardiac denervation as assessed by MIBG imaging despite a preserved left ventricular systolic function and cardiac perfusion, without correlation with conduction disturbances. Results of the heart rate variability analysis were more variable and this technique does not seem to be the best way to evaluate the extent of cardiac sympathetic denervation in FAP patients.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1617-4623
    Keywords: Key wordsCandida albicans ; Green fluorescent protein ; Virulence gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetically engineered versions of the GFP gene, which encodes the green fluorescent protein of Aequorea victoria, were placed under the control of the constitutively active Candida albicansACT1 promoter and integrated in single copy into the genome of this pathogenic yeast. Integrative transformants in which one of the two ACT1 alleles had been replaced by a GFP gene exhibited a homogeneous, constitutive fluorescent phenotype. Cells expressing GFP with the wild-type chromophore exhibited very weak fluorescence compared to those GFP proteins with the S65T or S65A, V68L, S72A (GFPmut2) chromophore mutations. Substitution of the CTG codon, which specifies serine instead of leucine in C. albicans, by TTG was absolutely necessary for GFP expression. Although GFP mRNA levels in cells containing a GFP gene with the CTG codon were comparable to those of transformants containing GFP with the TTG substitution, only the latter produced GFP protein, as detected by Western blotting, suggesting that the frequent failure to express heterologous genes in C. albicans is principally due to the non-canonical codon usage. Transformants expressing the modified GFP gene from the promoter of the SAP2 gene, which encodes one of the secreted acid proteinases of C. albicans, showed fluorescence only under conditions which promote proteinase expression, thereby demonstrating the utility of stable, chromosomally integrated GFP reporter genes for the study of gene activation in C. albicans.
    Type of Medium: Electronic Resource
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