ISSN:
1573-6849
Keywords:
fluorochrome staining
;
heterochromatin
;
in situ hybridization
;
Mytilus galloprovincialis
;
nucleolar organizing regions
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The chromosomes of the mussel Mytilus galloprovincialis were analysed by means of chromomycin A3 (CMA), distamycin A/DAPI (DA/DAPI), DAPI/actinomycin D (DAPI/AMD) and chromomycin A3/distamycin A/DAPI(CDD) fluorescence banding techniques, C-banding, silver staining, N-banding and in situ hybridization with 18S+28S rDNA and telomere probes. 18S+28S rDNA clusters were located on the telomeres of two pairs of submeta/subtelocentric chromosomes. The nucleolar organizing regions (NORs) were associated with bright CMA fluorescence, dull DAPI fluorescence and C- and N-positive bands, but not all four NOR-associated heterochromatin bands showed bright CMA fluorescence in a given cell; intra- and interindividual variability was found in this character. Additional non-ribosomal C-bands did not show any differential fluorescent behaviour.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018475804613
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