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  • 1995-1999  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the PKC-γ-Related Immunocrossreactive Region of a Novel Leukocyte Protein γ-rp (1999)
    Springer
    Inflammation 23 (1999), S. 387-410 
    Details
    ISSN: 1573-2576
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The newly discovered gamma-PKC-related-protein of human leukocytes (γ-rp) crossreacts with a polyclonal antibody preparation originally designed to be specific for PKC-γ (γMb-Ab). As this antibody is currently the only suitable probe for γ-rp, we sought to characterize the binding of the two proteins. We determined that the γMg-Ab does not recognize the native form of γ-rp. However, with denaturing immunoblots of γ-rp, we found that 1) the crossreactive γ-rp epitope differs somewhat from that of classic rat brain PKC-γ, but probably only to the degree of the rat/human PKC species difference; 2) the previously reported doublet bands of γ-rp represent a single protein with cell-stimulus inducible modifications; 3) antibodies present in the γMg-Ab pool bind to two separate sites within the γ-rp epitope; 4) access to one binding site is conformationally restricted, even after protein denaturation; 5) agonist-induced modification of γ-rp does not significantly affect the total amount of γMg-Ab that it can bind, but 6) does significantly affect the rate of antibody binding to one site. This investigation defines the appropriate experimental use of our antibody, and the significance of these findings for the future study and cloning of γ-rp is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020265701407
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    An Apparently Novel Protein of Human Leukocytes, Reactive with an Antibody to Protein Kinase C-γ, Is Rapidly Modified upon Cell Activation: Initial Characterization in Neutrophils and Their Cytoplasts (1998)
    Springer
    Inflammation 22 (1998), S. 1-28 
    Details
    ISSN: 1573-2576
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract On immunoblots of human neutrophil cytoplasts (U-CYT), a previously undescribed 97kDa protein was revealed by intense and selective reaction with an antibody that was initially raised to recognize PKC-γ. Denoted “γ-rp” for gamma-related protein, this acidic cytosolic protein somewhat resembled the classic forms of PKC in several biochemical respects. Appearing as a doublet on low-percentage SDS-PAGE gels, both its mobility and staining pattern were rapidly altered by treatment of U-CYT with either phorbol ester or chemotactic peptide. Whole neutrophil γ-rp was detectable only after TCA precipitation of intact cells. It was also detectable in human platelets, lymphocytes, and neutrophil-like differentiated HL60 cells, but not in fibroblasts, erythrocytes, monocytes, or monocyte-like differentiated HL60 cells. Our data suggest that γ-rp merits further study as a potential participant in cellular activation, and as a possible structural or functional relative of PKC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022390406225
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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