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  • 1
    Electronic Resource
    Electronic Resource
    Rapid regeneration of whole plants in large crabgrass (Digitaria sanguinalis L.) using thin-cell-layer culture (1998)
    Springer
    Plant cell reports 18 (1998), S. 166-172 
    Details
    ISSN: 1432-203X
    Keywords: Key words Transverse thin cell layer ; Monocot ; Pseudo-embryogenic structure ; Whole plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method was designed to produce rapidly (10–14 days) and directly (without intermediate callus) whole plants of Digitaria sanguinalis with a high yield without subculture. These plants developed from new structures, designated “pseudo-embryogenic structures”, initiated only 1 week after the culture of tranverse thin cell layers (tTCLs), i.e., thin stem sections, on a Murashige and Skoog medium containing 3% sucrose and a combination of a low concentration of 2,4-dichlorophenoxyacetic acid (1 μm) and a high concentration of 6-benzylaminopurine (10 μm). The fresh weight of plants regenerated per tTCL on gelrite was 6 times higher than with agar and 30 times higher than with agarose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050551
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and oplant regeneration in Sorghum bicolor (L.) Moench (1996)
    Springer
    Plant cell reports 15 (1996), S. 900-904 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: 2,4‐D: 2,4 dichlorophenoxyacetic acid, 2iP: N6‐(2‐isopentyl)adenine, BAP: 6‐benzylaminopurine, CaMV: cauliflower mosaïc virus, CPPU: N‐(2‐chloro 4‐pyridil)‐N'‐phenylurea, dicamba: 3,6‐dichloro‐o‐anisic acid, IAA: indole‐3‐acetic acid, K: kinetin, MS: Murashige and Skoog, NAA: α‐naphtaleneacetic acid, PEPC: phosphoenolpyruvate carboxylase, SD: standard deviation, tTCL: transverse thin cell layer.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. Callus induction, somatic embryogenesis and plant regeneration were obtained in two cultivars of Sorghum bicolor (L.) Moench. Transverse thin celllayers from roots/epicotyls of 15‐day‐old seedlings or of regenerated plantlets were used. Callus response depended on the genotype, the size of transverse thin cell layers, the level at which transverse thin cell layers were excised on the epicotyl, the composition of growth substances and the number of in vitro regeneration cycles undergone by the donor planet. Somatic embryos were differentiated under a defined dark/light sequence, from epidermised compact calluses (i.e. having already differentiated an epidermis), obtained directly with dicamba or from friable callus initiated with kinetin and 2,4 dichlorophenoxyacetic acid. The importance of kinetin and dicamba on the induction of embryogenic potential is reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050145
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Determination of endosulfan and some pyrethroids in waters by micro liquid-liquid extraction and GC-MS (1998)
    Springer
    Fresenius' journal of analytical chemistry 360 (1998), S. 568-572 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A simple, rapid and reproducible method for the determination of some pesticide residues in water was developed using micro liquid-liquid extraction and gas chromatography - mass spectrometry with selected ion monitoring (GC/MS-SIM). The chlorinated insecticides α- and β-endosulfan and endosulfan-sulfate as well as the synthetic pyrethroids bifenthrin, permethrin, cypermethrin, fenvalerate and deltamethrin can be separated from a 500 mL sample water extracted with 0.5 mL of n-hexane containing anthracene-d10 as internal standard without clean-up in only 13 min. The recovery efficiencies of the tested compounds yielded more than 93.0% at a fortification level of 5 ng mL–1 and their relative standard deviations were between 1.9 and 11.7%. Detection limit of each compound ranged between 3 and 35 pg mL–1. The method was applied to ground, sea and tap waters from Almería (Spain). The solubilities in water at 20° C were determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050760
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  • 4
    Electronic Resource
    Electronic Resource
    Perineurioma (storiform perineurial fibroma) of the kidney in a child (1998)
    Springer
    European radiology 8 (1998), S. 770-771 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Perineurioma ; Nerve sheath tumor ; Computed tomography ; Ultrasound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Perineurioma is an extremely rare benign tumor of the peripheral nervous system composed exclusively of perineurial cells. Imaging findings of this tumor are non-specific and the diagnosis is based on histologic studies. We report a case of perineurioma of the kidney in a 7-year-old girl discovered incidentally during the evaluation of a urinary tract infection. This is the first case of renal perineurioma reported in a child. We present the ultrasound and computed tomography findings of this histologically confirmed neoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050470
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  • 5
    Electronic Resource
    Electronic Resource
    Adaptation to gradual as compared with sudden visuo-motor distortions (1997)
    Springer
    Experimental brain research 115 (1997), S. 557-561 
    Details
    ISSN: 1432-1106
    Keywords: Key words Visuomotor learning ; Internal models ; Visual feedback rotation ; Motor control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  If visual feedback is discordant with movement direction, the visuo-motor mapping is disrupted, but can be updated with practice. In this experiment subjects practiced discrete arm movements under conditions of visual feedback rotation. One group was exposed to 10°-step increments of visual feedback rotation up to a total of 90°, a second group to a 90° visual feedback rotation throughout the experiment. After the first group reached the 90° visual feedback rotation, its subjects performed faster, with less spatial error, and showed larger aftereffects than the subjects who practiced constantly under the 90° visual feedback rotation condition. Results suggest that gradually increasing feedback distortion allows more complete adaptation than a large, sudden distortion onset.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00005727
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration in Sorghum bicolor (L.) Moench (1996)
    Springer
    Plant cell reports 15 (1996), S. 900-904 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Callus induction, somatic embryogenesis and plant regeneration were obtained in two cultivars of Sorghum bicolor (L.) Moench. Transverse thin cell layers from roots/epicotyls of 15-day-old seedlings or of regenerated plantlets were used. Callus response depended on the genotype, the size of transverse thin cell layers, the level at which transverse thin cell layers were excised on the epicotyl, the composition of growth substances and the number of in vitro regeneration cycles undergone by the donor plant. Somatic embryos were differentiated under a defined dark/light sequence, from epidermised compact calluses (i.e having already differentiated an epidermis), obtained directly with dicamba or from friable callus initiated with kinetin and 2,4 dichlorophenoxyacetic acid. The importance of kinetin and dicamba on the induction of embryogenic potential is reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231584
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Evidence for an electrogenic, negatively protein-kinase-A-modulated, Na+-dependent HCO3 – transporter in human lymphocytes (1999)
    Springer
    Pflügers Archiv 437 (1999), S. 935-943 
    Details
    ISSN: 1432-2013
    Keywords: Key words Bicarbonate ; Lymphocytes ; pHi regulation ; PKA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We studied the effects of external HCO3 – on pHi regulation in human lymphocytes after an acid load. Cells were acidified by preincubation with NH4Cl and pHi recovery was measured with the fluorescent dye BCECF. Cells recovering in HCO3 –-containing medium reached a higher final pHi, the H+ efflux rate was increased and shifted to alkaline pHi compared to that of cells recovering in HCO3 –-free solution. The resting pHi was higher in a HCO3 –-containing solution. Experiments carried out in the presence of amiloride, DIDS and in the absence of external Na+ suggest the existence of two major mechanisms acting in the pHi recovery of lymphocytes after an acid load: an amiloride-sensitive Na+/H+ exchanger and a DIDS-sensitive Na+-dependent HCO3 –transporter. The last mechanism could be a Na+/HCO3 –cotransporter based on membrane potential changes determined with the potential-sensitive fluorescent probe bis-oxonol. Preincubation of cells with forskolin and H-89 showed protein-kinase-A-dependent downregulation of the amiloride-insensitive recovery of pHi in human lymphocytes. In summary, this paper provides functional evidence for the existence of a Na+/HCO3 –-dependent mechanism involved in pHi recovery in human lymphocytes following an acid load, that is electrogenic and downregulated by PKA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050865
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  • 8
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis on Thin Cell Layers of a C4 species, Digitaria sanguinalis (L.) Scop (1997)
    Springer
    Plant cell, tissue and organ culture 49 (1997), S. 201-208 
    Details
    ISSN: 1573-5044
    Keywords: transverse thin cell layer ; monocot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryogenesis was obtained from transverse thin cell layers (tTCLs) of Digitaria sanguinalis. tTCLs (0.2 - 0.4mm thick, 1mm in diameter) were excised from 4-week-old seedlings and placed onto Murashige and Skoog media supplemented with a varying concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) (from 1 µM to 100 µM) and sucrose (from 3% to 24%). Somatic embryos were obtained in the dark 7-10 days after inoculation from tTCLs excised at specific levels on the seedling and cultured in the presence of 2,4-D (5 µM to 10 µM) and sucrose (3 to 6%). The exposure of the tTCLs to light decreased the percentage of tTCLs forming somatic embryos. Viable plantlets were obtained 2 weeks after transfer onto a cytokinin-containing medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005816126824
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Differentiation of Clams from Fishing Areas as an Approximation to Coastal Quality Assessment (1999)
    Springer
    Environmental monitoring and assessment 54 (1999), S. 229-237 
    Details
    ISSN: 1573-2959
    Keywords: coastal index ; discriminant analysis ; fishing areas ; heavy metals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The quality assessment of fishing areas on the basis of the levels of heavy metals in clams ( Chamelea gallina) was attempted by using discriminant analysis. Five metals, Hg, Pb, Cd, Cu and Zn, were analyzed in the soft edible parts of clams from three fishing areas. The descriptive methods applied to data obtained do not show enough differences between sampling stations for management purposes. Only discriminant analysis is successful in the differentiation between all fishing areas. Through the first discriminant function, the group centroids are proposed as index of different source of clams. These values standardized are proposed as coastal quality index.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005960212211
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  • 10
    Electronic Resource
    Electronic Resource
    HPLC determination of pesticides in green bean samples after SPE clean-up (1996)
    Springer
    Chromatographia 43 (1996), S. 265-270 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Diode array detection ; Solid-phase extraction (SPE) ; Pesticide analysis ; Multiresidue methods ; Green bean samples
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A reversed-phase high performance liquid chromatographic (HPLC) method with an acetonitrile-methanol-water mobile phase gradient and photodiode-array detection (DAD) is described for simultaneous determination of 21 pesticides, frequently used in agriculture, of different types, namely organophosphorus, organochlorine,N-methylcarbamates, triazines and phenylureas in vegetable samples. The pesticides were extracted with acetone and then partitioned from the vegetable sample with dichloromethane. Sample clean-up was accomplished by solid-phase extraction (SPE) using both C18 and florisil SPE columns. Average recoveries from green beans ranged from 70.0 to 110.1%. Detection limits of less than 0.1 mg kg−1 were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02270992
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