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  • 1
    Electronic Resource
    Electronic Resource
    Effect of exogenous auxin on root morphology and secondary metabolism in Tagetes patula hairy root cultures (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 93 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this paper the effects of indole-3-acetic acid (IAA) on growth of Tagetes patula hairy root cultures and secondary product formation are presented. The biosynthesis of thiophenes, sulfurous compounds with nematicidal activity, was inhibited by IAA application, as was evident from a decrease of [35S] sulfur incorporation. The inhibition only occurred after the roots had developed numerous laterals as a result of auxin action. However, in roots cultured in the absence of IAA, there was no significant correlation between branching and thiophene accumulation. Therefore, development of lateral roots is not a sufficient condition for a low capacity to synthesize thiophenes. The highest rate of thiophene accumulation in the roots culture is at its maximum. Hence, growth and the production of thiophenes appear to be compatible in T. Patula hair roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb02222.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Thiophene interconversion in elicitor-treated roots ofTagetes patula L. (1995)
    Springer
    Plant cell reports 15 (1995), S. 133-137 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thiophenes are polyacetylene-related heterocyclic metabolites. Some of these compounds are phototoxic, but the bithiophenes occurring inTagetes mainly accumulate in the root where photo-activation is not likely to occur. A cell-free extract from the fungusFusarium oxysporum induced biosynthesis of hydrophilic thiophenes in root cultures and roots of seedlings ofTagetes patula. The thiophenes formed were partially excreted into the culture medium. The excreted thiophenes inhibited fungal growth in the absence of light and thus may play a role in the biochemical defense against pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690270
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Hydration-dependent gene expression in Brassica oleracea anthers (1999)
    Springer
    Sexual plant reproduction 12 (1999), S. 135-143 
    Details
    ISSN: 1432-2145
    Keywords: Key words Brassica oleracea ; Drought-induced gene expression ; Programmed anther dehydration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Anther physiology is characterized by developmentally controlled dehydration. Screening of an anther cDNA expression library with an antiserum raised against proteins isolated from the surface of Brassica oleracea pollen grains resulted in the isolation of bopc15 and bopc34, sequences similar to water stress-inducible genes. Bopc15 transcripts were exclusively present in pollen. The mRNA of bopc34 was found in various tissues, but not in pollen. The accumulation of both transcripts preceded anther dehydration. Premature anther dehydration induced by imposed drought stress affects gene expression in both the sporophytic and gametophytic portions of the anthers the levels of bopc15 and bopc34 transcripts increased to an extent dependent on developmental stage and duration of treatment. The inducible bopc15 transcript accumulation was reversible by rehydration. Bopc34 transcript levels, on the other hand, do not follow this pattern.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050184
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a microspore-specific gene from tobacco (1996)
    Springer
    Plant molecular biology 31 (1996), S. 213-225 
    Details
    ISSN: 1573-5028
    Keywords: anther ; in situ hybridization ; microspore development ; microspore-specific gene ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The characterization of a gene with a unique microspore-specific expression pattern is reported. Isolated microspores from tobacco were used to synthesize a cDNA library. Clones that did not hybridize to leaf cDNA were further characterized by northern analysis. One clone proved to be a microspore-specific cDNA, representing a transcript of 650 nt. The corresponding gene, NTM19 (Nicotiana tabacum microspore-specific), was isolated and its sequence analysed. The gene encodes a protein of 10.8 kDa with a pI of 6.92 and a putative signal sequence at the N-terminus. A localization study revealed a unique spatial and temporal distribution. The transcript was only detected in the unicellular microspore. No hybridization signals were observed in other pollen developmental stages, nor in the surrounding anther tissues or other vegetative tissues of the plant. Therefore it can be concluded that NTM19 is a gene with a highly microspore-specific character according to both localization and stage of expression. Southern blot analysis demonstrated the presence of a small gene family. The occurrence of TNM19 was investigated in a range of closely and distantly related species and was found to be present in other solanaceous species, including the ancestors of tobacco and in a monocot species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021785
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular and metabolic control of secondary metabolism (1995)
    Springer
    Plant cell, tissue and organ culture 43 (1995), S. 127-130 
    Details
    ISSN: 1573-5044
    Keywords: partitioning ; root cultures ; sulphur ; Tagetes ; thiophenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Secondary metabolism is restricted to specific places in the plant. The concentrations of precursors and end products are the determining factors in the metabolic control of synthesis and breakdown of the compounds involved. Molecular control operates at the level of enzyme amount and gene expression. If the secondary product contains an element in its molecule which is derived from a mineral nutrient in the environment, the operation of the control mechanisms can be studied by varying the concentration of that mineral. This is exemplified by thiophene metabolism in root cultures ofTagetes. The characteristic groups in the molecule are two five-membered rings with a sulphur atom. In the experiments, the rate of thiophene biosynthesis was manipulated by varying the sulphate concentration in the medium. Sulphur limitation led to preferential channeling of sulphur into primary metabolism and a concomitant drop in thiophene biosynthesis. The major part of the reduction was caused by a drop in enzyme activity. Substrate availability played a minor role. The results indicate that sulphur is involved in the molecular control of secondary metabolism inTagetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00052167
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    Paper (German National Licenses)
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