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  • 1
    Electronic Resource
    Electronic Resource
    Cloning and Characterization of a Mouse σ1 Receptor (1998)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 70 (1998), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: A cDNA clone (S2-1a) isolated from a mouse brain cDNA library, using a guinea pig σ1 cDNA as probe, has high homology to the predicted protein sequence of the guinea pig (88%) and human (90%) σ1 receptors. Northern analysis revealed a major mRNA of ∼1.8 kb in a wide range of mouse tissues, with highest levels in brain, liver, kidney, and thymus. Southern analysis and chromosomal mapping in the mouse suggested a single-copy gene in region A5-B2 of chromosome 4. Expression of the clone in MCF-7 and CHO cells led to a pronounced increase in (+)-[3H]pentazocine binding with a selectivity profile consistent with σ1 receptors. In vitro translation yielded a protein of ∼28 kDa, as did transfection of a probe containing the hemagglutinin (HA) epitope (S2-1a.HA) into CHO cells, as determined by western analysis using an antibody directed against HA. (+)-[3H]-Pentazocine binding to immunopurified HA-tagged receptor demonstrated conclusively that S2-1a.HA encodes a high-affinity (+)-[3H]pentazocine binding site with characteristics of a murine σ1 receptor. An antisense oligodeoxynucleotide designed from S2-1a potentiated opioid analgesia in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1998.70062279.x
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  • 2
    Electronic Resource
    Electronic Resource
    Derivatives of the Gravity Potential with Respect to Rectangular Coordinates (1998)
    Springer
    Celestial mechanics and dynamical astronomy 71 (1998), S. 137-151 
    Details
    ISSN: 1572-9478
    Keywords: gravity potential ; derivatives ; rectangular coordinates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Starting from a complex operator of derivation, we give expressions for derivatives of arbitrary order of the gravity potential with respect to rectangular coordinates. These expressions have a form similar to the original potential expanded in spherical harmonics and are free of singularity at the poles. Computing sets of numerical coefficients once for all, we can compute the derivatives with a very limited work: the same functions are used to compute all derivatives by means of a unique parametrized formula. This is very comfortable for further algebraic manipulations. Numerical tests prove the accuracy and the efficiency of the algorithm derived from our formula to compute the gravity acceleration vector and the gravity gradient tensor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008361202235
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation of YBa2Cu3O7 − δ Thin Films by the Self-Template Sputtering Method (1998)
    Springer
    Journal of superconductivity 11 (1998), S. 737-741 
    Details
    ISSN: 1572-9605
    Keywords: High-temperature superconductor ; thin film ; YBaCuO
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract Using the self-template technique, c-axis-oriented epitaxial YBa2Cu3O7 − δ thin films have been prepared in situ on LaAlO3 substrate by the d.c. magnetron sputtering method. The properties of thin film dependence on the deposition conditions of the two-step self-template method have been systematically investigated. By optimizing the parameters, high-quality YBCO thin films with T c0 ≥ 90 K, ΔT c ≤ 1 K, R s (77 K, 10 GHz)∼500 μΩ were reproducibly obtained. The best sample grown under optimal conditions gave a low R s of 330 μΩ at 77 K, 10 GHz, which can be used in a microwave field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022645224036
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of a human stromal cell line supporting hematopoietic progenitor cell proliferation: Effect of HIV expression (1995)
    Springer
    Journal of biomedical science 2 (1995), S. 330-342 
    Details
    ISSN: 1423-0127
    Keywords: CD34+ cells ; Stromal cells ; Cytokines ; Human immunodeficiency virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Our objective was to determine the role that bone marrow-derived stromal cells have on human hematopoiesis in HIV infection. In particular, we dissected the heterogeneous bone marrow microenvironment to study the effect HIV expression might have on the cell population capable of producing the cytokines which will support human CD34+ cell differentiation. A stromal cell line, Lof(11-10), was established from human bone marrow by transfecting a plasmid containing the SV40 large T-antigen and isolating foci exhibiting a transformed phenotype. The Lof(11-10) cell line was characterized to determine its susceptibility to HIV infection, to identify its cytokine production profile, and to test the ability of conditioned media from this line to support CD34+ cell differentiation in the presence and absence of HIV expression. Nine cytokines were detected by RT-PCR and ELISA analysis. Conditioned media obtained from the Lof(11-10) cell line was able to support CD34+ cell differentiation. However, because the Lof(11-10) cells are not infectible by HIV, molecular clones of HIV were introduced into these cells by transfection. There was no qualitative difference in the levels of cytokine production between HIV-expressing and control Lof(11-10) cells. Furthermore, conditioned media derived from HIV-expressing and control Lof(11-10) cells added to bone marrow-derived CD34+ progenitor cells yielded similar colony formation in methylcellulose assays. Our data suggest that HIV infection of the cytokine-producing cells within the bone marrow microenvironment, as represented by the Lof(11-10) cell line, results in both normal cytokine production and hematopoiesis in spite of HIV expression. This report adds to the evidence against stromal cells being a significant target of HIV and establishes a system for comparison with more relevant models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02255220
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  • 5
    Electronic Resource
    Electronic Resource
    Sensitive determination of nucleotides and polynucleotides based on the fluorescence quenching of the Tb3+-Tiron complex (1997)
    Springer
    Fresenius' journal of analytical chemistry 358 (1997), S. 514-518 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract  A sensitive method using fluorescence quenching for the determination of nucleotides (ATP, ADP, AMP, CTP, UTP) and polynucleotides[poly(A), poly(I), poly(U)] is proposed. It is based on the ability of nucleotides and polynucleotides to inhibit the formation of a strongly fluorescent complex of Tb3+ ion with Tiron. The possibilities of spectrofluorimetric measurements of these systems were studied under optimal conditions (pH 6.9 in hexamethylene tetramine-HCl buffer, 1.2×10-6 mol/L of Tb3+, 4.0×10-6 mol/L of Tiron, λex=317 nm, λem=546 nm). The results showed that the Tb3+-Tiron complex could be used as a fluorescence test for the phosphate moieties of nucleotides and polynucleotides. The detection limits are 0.3, 1.2, 3.7, 0.2, 0.3, 1.1, 0.6 and 0.9 ng/mL for ATP, ADP, AMP, CTP, UTP, poly(A), poly(I), and poly(U), respectively. The relative standard deviations (6 replicates) are within 4.0% in the middle of the linear range. The fluorescence quenching mechanism of these systems is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050457
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  • 6
    Electronic Resource
    Electronic Resource
    Synchronous Fluorescence Spectrometric Methodology in the Wavelength Domain (1999)
    Springer
    Journal of fluorescence 9 (1999), S. 173-179 
    Details
    ISSN: 1573-4994
    Keywords: Spectrofluorimetry ; synchronous fluorescence spectra ; peak maximum ; bandwidth ; variable-angle synchronous scanning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Theoretical relationships suitable for both constant-wavelength and variable-angle synchronous fluorescence in the wavelength domain were obtained for the calculation of peak location, intensity, and half-width of synchronous fluorescence spectra. The calculated values were compared with experimental data and the data derived from the literature equations, indicating that the proposed methods are feasible, less formidable, and more straightforward in the wavelength domain than previous ones. The proposed approaches provide a theoretical guide for the experimental design of synchronous fluorescence spectrometric methods in the wavelength domain. On the basis of the theoretical derivation, special characteristics of the peak half-bandwidths of the spectra were revealed. In certain cases, the bandwidth of a variable-angle synchronous spectrum is of less practical meaning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022547315162
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  • 7
    Electronic Resource
    Electronic Resource
    The chromatic polynomial between graph & its complement—about Akiyama and Hararys' open problem (1995)
    Springer
    Graphs and combinatorics 11 (1995), S. 337-345 
    Details
    ISSN: 1435-5914
    Keywords: chromatic polynomial ; self-complementary graph ; self-complementary degree sequence graph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract In 1980, Akiyama and Harary proposed the following problem in the Proceedings-Fourth International Graph Theory Conference: “Are there any graphsG which are not self-complementary withG and $$\bar G$$ having the same chromatic polynomial?”. The problem has been unsolved until now. This paper proves that there are no graphsG which are not self-complementary withG and $$\bar G$$ having the same chromatic polynomial when |V(G)| =p 〈 8 orp = 2, 3 (mod 4), there must be a graphG which are not self-complementary withG and $$\bar G$$ having the same chromatic polynomial when |V(G)| =p ≥ 8 andp ≡ 0, 1 (mod 4).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01787814
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of Sputtering Parameters on Superconducting Properties of GdBaCuO Epitaxial Thin Film Deposited by ICP Method (1998)
    Springer
    Journal of superconductivity 11 (1998), S. 317-322 
    Details
    ISSN: 1572-9605
    Keywords: Superconductor ; thin film ; GdBaCuO
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract Thin films of GdBaCuO (GBCO) have been deposited in situ onto LaAlO3 single crystal substrates by inverted cylindrical sputtering pattern (ICP). The superconductive properties of the thin films' dependence on the substrate temperature and sputtering pressure have been systematically investigated. By optimization of the deposition parameter, high-quality c-axis epitaxial GBCO thin films of T c0〉92 K were reproducibly grown. The T c of the best sample is as high as 93.2 K. Upon changing the target composition to GdBa2Cu4O y (Gd124), it was observed that the samples always show some a-axis oriented films, implying that excess copper would favor a-axis growth in thin films. The superconductivity of the thin films under higher substrate temperature (T s〉800°C) was clearly improved by the procedure of special post-oxygenization at 400°C with an ozone atmosphere. This is very useful for preparing large-area thin films of GBCO.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022652505611
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  • 9
    Electronic Resource
    Electronic Resource
    Full-Wave Analysis of Inside Cylindrical Microstrip Lines by Finite Element Method (1999)
    Springer
    International journal of infrared and millimeter waves 20 (1999), S. 1339-1352 
    Details
    ISSN: 1572-9559
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In this paper, a full-wave analysis using finite element method is presented to investigate the dispersion characteristics of inside cylindrical microstrip lines, which are essential to the design of some conformal microstrip circuits in millimeter wave bands. Numerical results of frequency-dependent effective dielectric constants are given for different structural dimensions and dielectric constants of substrate. Some useful conclusions are derived for the design of the special conformal microstrip lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1021722811268
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of two coupled radial line transformers in dual-diode millimeter wave oscillators (1995)
    Springer
    International journal of infrared and millimeter waves 16 (1995), S. 921-934 
    Details
    ISSN: 1572-9559
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Two mutually coupled radial line transformers mounted in a common rectangular waveguide of dual—diode millimeter wave oscillator are characterized by a hybrid technique based on Galerkin's method combined with collocation technique. The external circuit impedances looking outside from each of two active devices are calculated. A large number of numerical results of the external circuit impedances as a function of the structural parameters have been obtained. Some original conclusions have been made, which are helpful to the design and the adjustment of the dual—diode oscillators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02066666
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