ISSN:
1573-6903
Keywords:
Seizure
;
epilepsy
;
70-kDa protein
;
glycoprotein
;
cerebral cortex
;
rat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Using ion exchange HPLC and ammonium sulfate precipitation, we have purified a 70-kDa protein (P70) specific to the cobalt-induced epileptogenic cortex of rat cerebrum and determined certain of its biochemical properties. P70 has a similar isoelectric point (pI; 4.6–4.8), amino acid composition and N-terminal amino acid sequence to rat serum albumin (RSA). Intracortical application of purified P70 to the motor area of normal rat cerebrum induces both ECoG seizure discharges and behavioral seizures. The data suggest that P70 is a novel albumin-like protein linked to the generation of seizure activities. However, it can be clearly distinguished from RSA, since it is able to produce seizure, is a glycoprotein and can be readily separated from RSA by 2-dimensional electrophoresis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00970735
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