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  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of ethylene biosynthesis by antisense ACC oxidase RNA prevents chilling injury in Charentais cantaloupe melons (1999)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 22 (1999), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Non-freezing low temperature storage causes injury to melons and most other fruit and vegetables of tropical and subtropical origin. We demonstrate here that ethylene suppression through an antisense ACC oxidase (ACO) gene considerably reduced the sensitivity of Charentais cantaloupe melons to chilling injury. In contrast to wild-type fruit, antisense ACO melons did not develop the characteristic chilling injury of pitting and browning of the rind neither when stored at low temperature (3 weeks at 2 °C) nor upon rewarming. Treating antisense melons with 10 p.p.m. ethylene for more than 1 d prior to cold storage resulted in the restoration of chilling sensitivity. When the ethylene treatment was performed after cold storage, the chilling injury symptoms did not appear. The tolerance to chilling was associated with a lower accumulation of ethanol and acetaldehyde, reduced membrane deterioration and higher capacity of the fruit to remove active oxygen species. The activities of catalase, superoxide dismutase and peroxidase were markedly increased in antisense ACO fruit in comparison with wild-type fruit, particulary upon rewarming and post-storage ethylene treatment. Severe chilling injury symptoms were correlated with a lower activity of activated oxygen scavenging enzymes. These results demonstrate that ethylene acts in conjunction with low temperature to induce metabolic shifts that participate in the development of chilling injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.1999.00509.x
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of an antisense 1-aminocyclopropane-1-carboxylate oxidase gene stimulates shoot regeneration in Cucumis melo (1998)
    Springer
    Plant cell reports 17 (1998), S. 586-589 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsCucumis melo ; 1-Aminocyclopropane-1-carboxylate oxidase ; Ethylene ; Antisense RNA ; Transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of ethylene in shoot regeneration was investigated using transgenic Cucumis melo plants expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene. ACC oxidase catalyses the last step of ethylene biosynthesis. Leaf and cotyledon explants from the transgenic plants exhibited low ACC oxidase activity and ethylene production, whereas the regeneration capacity of the tissues was greatly enhanced (3.5- and 2.8-fold, respectively) compared to untransformed control tissues. Addition of ethylene released by 50 or 100 µm 2-chloroethylphosphonic acid dramatically reduced the shoot regeneration rate of the transgenic tissues. The results clearly demonstrate that ethylene plays an important role in C. melo morphogenesis in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050447
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.) (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 81-90 
    Details
    ISSN: 1617-4623
    Keywords: Key words ACC oxidase ; Ethylene ; Multigene family ; Differential expression ; Cucumis melo L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants, converting 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene. We have previously described the isolation and characterization of a cDNA clone (pMEL1) encoding an ACC oxidase homolog from melon (Cucumis melo L.). Here we report the isolation and characterization of three genomic clones, corresponding to three putative members of the ACC oxidase gene family in melon. All are transcriptionally active. The sequences of these genes have been determined. One genomic clone (CM-ACO1), corresponding to the cDNA previously isolated, presents a coding region interrupted by three introns. Its transcription initiation site has been defined with RNA from ripe fruit and ethylene-treated leaves. The other two genes (CM-ACO2, CM-ACO3) have only two introns, at positions identical to their counterparts in CM-ACO1. The degree of DNA homology in the coding regions of CM-ACO2 and CM-ACO3 relative to CM-ACO1 is 59% and 75%, respectively. CM-ACO2 and CM-ACO3 are 59% homologous in their coding regions. These three genes have close homology to PH-ACO3, a member of the ACC oxidase multigene family of petunia. The predicted amino acid sequences of CM-ACO1 and CM-ACO3 are 77% to 81% identical to those encoded by the tomato and petunia genes, while the deduced amino acid sequence of CM-ACO2 shows only 42% to 45% homology. RT-PCR analysis using gene-specific primers shows that the three genes are differentially expressed during development, ethylene treatment and wounding. CM-ACO1 is induced in ripe fruit and in response to wounding and to ethylene treatment in leaves. CM-ACO2 is detectable at low level in etiolated hypocotyls. CM-ACO3 is expressed in flowers and is not induced by any of the stimuli tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174348
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  • 4
    Electronic Resource
    Electronic Resource
    Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.) (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 81-90 
    Details
    ISSN: 1617-4623
    Keywords: ACC oxidase ; Ethylene ; Multigene family ; Differential expression ; Cucumis melo L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants, converting 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene. We have previously described the isolation and characterization of a cDNA clone (pMEL1) encoding an ACC oxidase homolog from melon (Cucumis melo L.). Here we report the isolation and characterization of three genomic clones, corresponding to three putative members of the ACC oxidase gene family in melon. All are transcriptionally active. The sequences of these genes have been determined. One genomic clone (CM-ACO1), corresponding to the cDNA previously isolated, presents a coding region interrupted by three introns. Its transcription initiation site has been defined with RNA from ripe fruit and ethylene-treated leaves. The other two genes (CM-ACO2, CM-ACO3) have only two introns, at positions identical to their counterparts inCM-ACO1. The degree of DNA homology in the coding regions ofCM-ACO2 andCM-ACO3 relative toCM-ACO1 is 59% and 75%, respectively.CM-ACO2 andCM-ACO3 are 59% homologous in their coding regions. These three genes have close homology toPH-ACO3, a member of the ACC oxidase multigene family of petunia. The predicted amino acid sequences of CM-ACO1 and CM-ACO3 are 77% to 81% identical to those encoded by the tomato and petunia genes, while the deduced amino acid sequence ofCM-ACO2 shows only 42% to 45% homology. RT-PCR analysis using gene-specific primers shows that the three genes are differentially expressed during development, ethylene treatment and wounding.CM-ACO1 is induced in ripe fruit and in response to wounding and to ethylene treatment in leaves.CM-ACO2 is detectable at low level in etiolated hypocotyls.CM-ACO3 is expressed in flowers and is not induced by any of the stimuli tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174348
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Differential activation of two ACC oxidase gene promoters from melon during plant development and in response to pathogen attack (1997)
    Springer
    Molecular genetics and genomics 256 (1997), S. 211-222 
    Details
    ISSN: 1617-4623
    Keywords: Key words ACC oxidase ; Ethylene ; Hypersensitive ; Response ; Senescence ; Transcriptional activation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ACC (1-aminocyclopropane-1-carboxylate) oxidase genes are differentially expressed in melon during development and in response to various stresses. We investigated the molecular basis of their transcription by analyzing the 5′ untranslated regions of the ACC oxidase genes CM-ACO1 and CM-ACO3. In order to determine how their temporal and spatial expression patterns were established, we fused the promoter regions of CM-ACO1 (726 bp) and CM-ACO3 (2260 bp) to the β-glucuronidase (GUS) reporter gene and examined their regulation in transgenic tobacco plants. The CM-ACO1 promoter was able to drive GUS expression in response to wounding, and to treatment with ethylene or copper sulfate. It was also rapidly induced (8–12 h post-inoculation) in tobacco leaves inoculated with the hypersensitive response (HR)-inducing bacterium Ralstonia solanacearum. Expression was also observed during compatible interactions but was delayed. In contrast, the CM-ACO3 promoter was not expressed in response to infection, but was up-regulated during flower development. Both promoters were regulated during leaf senescence but in different patterns. The CM-ACO1-driven GUS activity increased sharply concomitantly with the onset of chlorophyll breakdown, while the CM-ACO3 promoter drove strong GUS expression in green, fully expanded leaves and this declined at the onset of senescence. This result is consistent with the expression patterns of these two genes in senescent melon leaves. These data suggest that the regulation of expression of CM-ACO1 is related preferentially to stress responses, whereas CM-ACO3 seems to be associated with developmental processes. The possible role of ethylene is discussed, particularly in the regulation of the CM-ACO1 gene in response to stress and during senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050563
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