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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 42 (1989), S. 163-170 
    ISSN: 1573-5060
    Keywords: Azolla ; fern ; isozymes ; electrophoresis ; biochemical taxonomy ; differentiation ; variation ; hybrid identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Enzyme electrophoresis was used to differentiate sections within the genus Azolla Lam. and demonstrate the value of this method in fingerprinting taxa. Polyacrylamide gel methodology has been developed to identify suspected species of this genus. Preliminary examination of selected isolates indicated that allozyme diversity was particularly evident in section Azolla, where specific classification by morphological means is difficult. Leaf tissue was preferable to root tissue for this purpose because of the ease of tissue preparation and the superior resolution and staining intensity of enzymes. Contribution of enzyme bands by the cyanobiont to zymograms was not significant.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 43 (1989), S. 223-232 
    ISSN: 1573-5060
    Keywords: Azolla ; classification ; fingerprinting ; isozymes ; taxonomy ; trichomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Azolla accessions (section Azolla) from the germplasm collections of the International Rice Research Institute and Washington State University were fingerprinted and classified by enzyme electrophoresis and leaf trichome morphology. A. filiculoides was enzymatically distinctive and also reliably identified by its prominent one-celled trichomes. Neotropical accessions labelled as A. filiculoides proved to be members of other species. Two groups of isolates were designated A. rubra, but those from Japan were identified as A. filiculoides. The A. rubra of Australia-New Zealand was biochemically unique and possessed less protuberant trichomes than A. filiculoides. A. microphylla, A. mexicana, and A. caroliniana were phenetically similar, but a. microphylla was identifiable from the others in the banding patterns of certain enzymes. A. mexicana and A. caroliniana were closely related enzymatically. The two-celled leaf trichomes of these three species were similar in size and shape.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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