ISSN:
1432-0584
Keywords:
Immune thrombocytopenic purpura
;
Microparticles
;
Antiphospholipid antibody
;
Flow cytometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary We recently reported that IgM antibody-related microparticles exist in some patients with idiopathic thrombocytopenic purpura (ITP) [14]. In this study, we investigated the relationship between antiphospholipid (cardiolipin and phosphatidylinositol) antibodies and microparticles in 56 ITP patients. We used an ELISA to detect anti-phospholipid antibodies. IgG antibodies against cardiolipin and phosphatidylinositol were detected in 13 and 12 patients, respectively. The titers of IgG antibodies against these phospholipids did not correlate with the platelet-associated IgG level or the platelet count. Next, we investigated the binding of anti-phospholipid antibodies to platelets and microparticles. Microparticles were obtained by incubating washed platelets with collagen plus thrombin. ITP plasma containing IgG-class anti-phospholipid antibodies showed significantly increase binding to microparticles compared with plasma without such antibodies (p〈0.001). Our results suggest that anti-phospholipid antibodies could affect the function of platelet microparticles in ITP.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01715126
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