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  • 1
    Electronic Resource
    Electronic Resource
    A histidine binding protein ofEscherichia coli: a component of cystine binding protein ofEscherichia coli (1993)
    Springer
    Amino acids 5 (1993), S. 39-50 
    Details
    ISSN: 1438-2199
    Keywords: Amino acids ; Histidine ; Cystine ; Amino acid binding proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Commercially obtained cystine binding protein (CBP), an osmotic shock protein ofEscherichia coli, was studied in an effort to determine its binding characteristics. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) analysis of commercially obtained CBP showed three protein bands. N-terminal amino acid microsequencing and subsequent computer search revealed that the sequence of one of these proteins (25-kDa) was nearly identical to histidine binding protein (HisJ) ofSalmonella typhimurium. Purification of CBP by HPLC yielded four protein peaks, of which one bound histidine exclusively. Binding was maximal at pH 5.0 to 6.0, at 4°C, did not require calcium or magnesium ions and was not inhibited by reduction of CBP disulfide bonds. Amino acids other than histidine or cystine did not bind to CBP. These data show that commercially available CBP is not a homogenous protein; it contains a histidine as well as a cystine binding component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00806191
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Association of HLA antigens with differential responsiveness toMycobacterium w vaccine in multibacillary leprosy patients (1992)
    Springer
    Journal of clinical immunology 12 (1992), S. 50-55 
    Details
    ISSN: 1573-2592
    Keywords: HLA ; lepromin conversion ; histopathological upgrading ; lepromatous leprosy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Leprosy patients undergoing phase II trials in two hospitals of New Delhi, India, were HLA typed to see the association of HLA with differential responsiveness toMycobacterium w vaccine. The vaccine comprises an atypical, nonpathogenic mycobacterium,Mycobacterium w, which has cross-reactive antigens withM. leprae. Multibacillary patients who are lepromin negative are vaccinated at an interval of 3 months. Considerable improvement is evident in the patients in terms of a decline in bacterial indices and histopathological and immunological upgrading. But all the patients do not respond to the vaccine in the same manner; some are slow responders, while others are good responders. HLA-A28 and DQw3 (DQw8+9) were found to be associated with slow responsiveness, while DQw1 and DQw7 were found to be associated with a more rapid responsiveness to theM. w vaccine. However, these associations were not significant afterP correction for the number of antigens tested for each locus except for HLA-DQw3 (DQw8 and DQw9) and DQw7. DQw7, a new defined split of HLA-DQw3, seems to be associated with the responsiveness toM. w vaccine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00918273
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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