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Comparison between the interaction of steroids with [35S]TBPS binding to cerebral cortical and to pituitary membranes: correlation with inhibition of prolactin release (1992)

Vincens, Monique ; Shu, Christine ; Fortin, Michel ; [et al.]

Springer

Naunyn-Schmiedeberg's archives of pharmacology 346 (1992), S. 523-526

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ISSN: 1432-1912

Keywords: Steroids ; [35S]TBPS binding ; Cortical membranes ; Prolactin ; Pituitary ; GABAA receptor complex

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary It has been shown previously that 5α-pregnan-3α-ol-20-one (5α3αP) can inhibit prolactin release from anterior pituitary gland cells in culture through an interaction with a specific modulatory site on the GABAA receptor complex in anterior pituitary gland membranes. In the present work, this receptor site has been labelled with [35S]t-butylbicyclophosphorothionate ([35S]TBPS) to enable a study of the relative binding affinities (RBA) of different steroids for the GABAA receptor complex to be made. We have found a high correlation (r = + 0.88) between the inhibition of [35S]TBPS binding to anterior pituitary membranes and the inhibition of [35S]TBPS binding to cerebral cortical membranes by nine different steroids. There was also a high correlation between the inhibition of prolactin release from anterior pituitary gland cells in culture by these steroids and the inhibition of [35S]TBPS binding to anterior pituitary membranes (r = + 0.99) or to cortical membranes (r = + 0.81). These observations suggest that the measurement of prolactin release from anterior pituitary gland cells in culture is a good indicator of the functional activity of drugs that bind to the allosteric modulatory TBPS-binding site on the GABAA-receptor complex.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00169007

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A method for finite element parallel viscous compressible flow calculations (1994)

Dutto, Laura C. ; Habashi, Wagdi G. ; Robichaud, Michel P. ; [et al.]

Chichester : Wiley-Blackwell

International Journal for Numerical Methods in Fluids 19 (1994), S. 275-294

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ISSN: 0271-2091

Keywords: Navier-Stokes ; Fully-coupled solution ; Iterative solvers ; Finite-element methods ; Parallel algorithm ; Block diagonal preconditioning ; CFD ; Engineering ; Engineering General

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: This paper presents the parallelization aspects of a solution method for the fully coupled 3D compressible Navier-Stokes equations. The algorithmic thrust of the approach, embedded in a finite element code NS3D, is the linearization of the governing equations through Newton methods, followed by a fully coupled solution of velocities and pressure at each non-linear iteration by preconditioned conjugate gradient-like iterative algorithms. For the matrix assembly, as well as for the linear equation solver, efficient coarse-grain parallel schemes have been developed for shared memory machines, as well as for networks of workstations, with a moderate number of processors. The parallel iterative schemes, in particular, circumvent some of the difficulties associated with domain decomposition methods, such as geometry bookkeeping and the sometimes drastic convergence slow-down of partitioned non-linear problems.

Additional Material: 15 Ill.