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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 17 (1983), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Human lymphocytes alloactivated in vitro were cloned by limiting dilution in the presence of filler cells and interleukin 2 (IL 2)-containing supernatants of phytohaemagglutinin-stimulated lymphocytes. Clones with allospecific proliferative reactivity (PLT clones), measured by tritiated thymidine (3H-TdR) incorporation, were selected for extensive IL 2-dependent expansion. The cloned lines had finite lifespans, ranging from an estimated minimum of 28 to 〉 65 doublings. Function as PLT reagents, however, was retained in all cases for only an estimated 30 cell doublings. This apparent cessation of function was not caused by loss of the ability to metabolize thymidine, since lines continuing to grow for 〉 30 doublings still incorporated 3H-TdR in the presence of IL 2. An altered requirement for stimulating antigen (number of stimulating cells), or altered response kinetics, did not contribute to loss of PLT function. Exogenous IL 2 added during restimulation to responders previously ‘rested’ overnight without IL 2 did not restore the response. Thus, under present experimental conditions, functional lifespans of cloned PLT reagents appear fixed at ˜ 30 cell doublings.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We have isolated a hybrid gene, composed of the first 455 nucleotides of hisP and nucieotides 275–1107 of malK, the genes coding for the nucleotide-binding components of the high-affinity transport systems for histidine and maltose in Salmonella typhimurium, respectively. The fusion had occurred by recombination within 11 homologous base pairs located between the two DNA fragments. In the chimeric protein peptidic motifs A and B, proposed to be part of the nucleotide-binding fold, originate from HisP and MalK, respectively. Plasmid pES42-39, harbouring the hybrid gene, was shown to complement only a malK mutation but failed to complement a hisP deletion mutation. The chimeric protein was identified by immunoblotting as a protein with an apparent molecular mass of 49kDa. Removal of the C-terminal 77 amino acid residues from the chimeric protein resulted in the loss of function in transport. In contrast, 51 amino acid residues could be removed from the C-terminus of wild-type MalK without any effect. Upon overproduction the chimeric protein, as wild-type MalK, inhibited expression of the malB regulon. However, both truncated proteins, when overproduced, did not exhibit this activity. Based on these results, a tentative model of the functional domains of MalK is presented.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome the DNA region located between gerB (314°) and sacXV (333°) was assigned to the Institut Pasteur. In this paper we describe the cloning and sequencing of a segment of 97 kb of contiguous DNA. Ninety-two open reading frames were predicted to encode putative proteins among which only forty-two were found to display significant similarities to known proteins present in databanks, e.g. amino acid permeases, proteins involved in cell wall or antibiotic biosynthesis, various regulatory proteins, proteins of several dehydrogenase families and enzymes II of the phosphotransferase system involved in sugar transport. Additional experiments led to the identification of the products of new B. subtilis genes, e.g. galactokinase and an operon involved in thiamine biosynthesis.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 9 (1982), S. 0 
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. The effect on renal function in sheep of inhibiting converting enzyme with captopril was examined before and after 5 days ACTH administration.2. Glomerular filtration rate, effective renal plasma flow, effective renal blood flow, mean arterial pressure and plasma sodium were all significantly incereased by ACTH treatment and plasma potassium was decreased. Captopril (20 mg i.v.) had no effect on renal function or blood pressure before or after ACTH treatment, although urinary potassium excretion decreased following captopril on day 6 of ACTH treatment.3. The increase in glomerular filtration rate and effective renal plasma flow seen with ACTH treatment in sheep does not appear to be mediated by the renin-angiotensin system.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 155 (1945), S. 176-176 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE note by N. I. Pinegin1 suggests a more detailed discussion of the relation between the threshold intensities for scotopic and photopic vision in the ultra-violet. The threshold ratio Tp/Ts is a measure of the intensity range, often misleadingly called ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of wildlife research 26 (1980), S. 126-132 
    ISSN: 1439-0574
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary In a wild boar enclosure in Austria, one individual several years old was observed smell marking and defending food. Apples were smell marked with urine and saliva. Young animals did not eat this food after having tested it by sniffing, although the marking ♂ did. The wild boar defended his feeding ground against men. In the process, he showed in a weaker or ritualised form behavioural patterns from the innate characteristics of parading, threatening and fighting. In addition, there is a report of an old boar in which acoustic impulses (the noises of a food container being used by another boar) triggered off a demonstration of rank and appropriation at the feeding ground. This trigger was more forceful than „avoidance of the enemy“. Mention is made of a case of imitation and learning in the overcoming of an obstacle.
    Abstract: Résumé Dans un parc à sangliers situé en Autriche un sanglier mâle adulte a été observé lorsqu'il procédait à des marquages olfactifs et lorsqu'il défendait sa nourriture. Des pommes furent marquées olfactivement au moyen d'urine et de salive. Contrairement au mâle en question, des sujets juvéniles n'ont pas absorbé cette nourriture apreast en avoir goûté par voie olfactive. Le verrat défendit son lieu de nourrissage contre les humains. A cette occasion il manifesta, sous une forme atténuée ou ritualisée, un comportement spécifique de défi, de menace et de combat. On fait également état d'une démonstration de rang social et d'appropriation sur une aire de nourrissage par un sanglier mâle âgé déclenchée par des stimuli acoustiques simulant le bruit provoqué par l'utilisation de mangeoires par d'autres sangliers. Ce déclencheur était plus puissant que l'„évitement d'un ennemi“. On signale un cas d'imitation et d'apprentissage lors du franchissement d'un obstacle.
    Notes: Zusammenfassung In einem österreichischen Wildschweingatter wurde ein mehrjähriger Keiler beim Duftmarkieren und Verteidigen von Futter beobachtet. Mit Harn und Speichel wurden Äpfel geruchlich markiert. Jungtiere haben dieses Futter nicht verzehrt, nachdem sie es olfaktorisch geprüft hatten, wohl aber das markierende ♂. Gegenüber Menschen verteidigte der Keiler seinen Futterplatz. Dabei zeigte er in abgeschwächter od. ritualisierter Form Verhaltensweisen aus dem innerartlichen Imponier-, Droh- und Kampfverhalten. Es wird ferner berichtet über einen alten Keiler, bei dem akustische Reize, die Geräusche eines von anderen Wildschweinen benutzten Futterbehälters, eine Rang- und Inbesitznahmedemonstration am Futterplatz auslösten. Dieser Auslöser war stärker als die „Feindvermeidung“. Ein Fall von Nachahmung und Lernen bei der Überwindung eines Hindernisses wird erwähnt.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 39 (1980), S. 233-238 
    ISSN: 1434-6036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The characteristic ferroelectric dispersion of the complex dielectric constant of the ferroelectric AgNa(NO2)2 is measured as function of temperature and hydrostatic pressure. For the first time the temperature and pressure dependences of the static order parameter susceptibility and the order parameter relaxation time could be reproducibly determined. The results are interpreted in terms of a kinetic Ising model, whose parameters show a characteristic pressure dependence. The results are discussed on the basis of thermodynamic relations and related to the dynamical behaviour of other order-disorder ferroelectrics.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied electrochemistry 22 (1992), S. 204-214 
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract One of the primary obstacles to be overcome for the development of economical fuel cells is the high cost of the membrane electrolyte. The currently favoured polymer electrolytes consist of poly(tetrafluoroethylene) backbone structures and poly(perfluorosulphonic acid) side chains. In an effort to find lower cost membranes, some radiation-grafted copolymer membranes were investigated. All the membranes contained poly(styrenesulphonic acid) side chains. Three different backbone polymer structures were studied: low-density poly(styrene), poly(tetrafluoroethylene)/poly(perfluoropropylene), and poly(tetrafluoroethylene). The results indicate that the membrane consisting of a poly(tetrafluoroethylene)/poly(styrenesulphonic acid) copolymer is a promising candidate as a fuel-cell electrolyte.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 296 (1980), S. 81-85 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The half-life of the 1/2− isomer in89Mo has been determined in an experiment where an enriched92Mo target was irradiated by 350 or 700 ms bursts of 60 MeV protons. Analysis of the gamma-ray spectra collected as a function of time between irradiations revealed gamma rays at 118.8 and 268.5 keV decaying with a 190±15 ms half-life. The hindrance ofE3 transitions inN=47 andZ=47 nuclides is discussed.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1440
    Keywords: TGF-β ; Anti-TGF-β ; Hematopoietic colony formation ; Peripheral blood
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of transforming growth factor-β (TGF-β) on hematopoiesis has been evaluated by adding blocking antibodies against TGF-β to colony forming assays (CFU-c). When optimum concentrations of recombinant growth factors, granulocyte-macrophage colony stimulating factor (GM-CSF), and interleukin-3 (IL-3) were added to stem cells from the peripheral blood of healthy individuals and certain patients with tumors or HIV infection, the anti-TGF-β capable of blocking 5 ng/ml of active TGF-β had no significant influence on erythroid or myeloid colony formation. However, in certain immunosuppressed individuals, anti-TGF-β resulted in a significant decrease of erythroid colony formation and slight suppression of myeloid colony formation. The significant inhibition of hematopoiesis by plasma of HIV patients could be due to the presence of active forms of TGF-β. The results of the blocking experiments are consistent with the concept that TGF-β in low concentrations is essential for erythropoiesis and myelopoiesis but that higher levels of TGF-β primarily inhibit erythropoiesis in vitro. TGF-β serves as a coordinating factor when efficient recruitment of granulocytes and monocytes is more essential than erythropoiesis and stem cell growth.
    Type of Medium: Electronic Resource
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