Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Homologous transformation of the lignin-degrading basidiomycete Phanerochaete chrysosporium (1991)
    Springer
    Current genetics 19 (1991), S. 491-494 
    Details
    ISSN: 1432-0983
    Keywords: Phanerochaete chrysosporium ; DNA transformation ; Basidiomycete ; Adenine biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A clone containing the Phanerochaete chrysosporium ade1 gene was isolated from a λEMBL3 genomic library using the ade5 gene encoding aminoimidazole ribonucleotide synthetase, from Schizophyllum commune, as a probe. A 6.0 kb fragment incorporating the ade1 gene was subcloned into pUC18 (pADE1) and used to transform the P. chrysosporium ade1 auxotrophic strain. Transformation frequencies were similar to those obtained previously with the S. commune ade5 gene; however, homologous transformants arose earlier than heterologous transformants. The transformants were mitotically and meiotically stable and Southern blot analysis indicated that the plasmid, pADE1, integrated ectopically in single or multiple copies. The pADE1 insert was mapped for restriction sites and the approximate location of the ade1 gene within the insert was determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312741
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium (1993)
    Springer
    Current genetics 23 (1993), S. 351-356 
    Details
    ISSN: 1432-0983
    Keywords: Phanerochaete chrysosporium ; DNA transformation ; Basidiomycete ; Uracil auxotrophs ; Homothallism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementation tests. The ura5 auxotrophs were transformed to prototrophy with the ura5 gene from the ascomycete Podospora anserina. The ura3 auxotrophs were transformed to prototrophy with the ura3 gene from the basidiomycete Schizophyllum commune. The P. chrysosporium ura3 gene was isolated from a γEMBL3 genomic library using the S. commune ura3 gene as a probe. A 6.6-kb fragment incorporating the ura3 gene was subcloned into Bluescript SK+(pURA3.1) and used to transform P. chrysosporium ura3 auxotrophic strains. The pURA3.1 insert was mapped for restriction sites and the approximate location of the ura3 gene within the insert was determined. Double auxotrophic strains were transformed with either of two marker genes and the resulting single auxotrophic strains were crossed to demonstrate genetic recombination between two nuclei of identical genetic background.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310898
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Chemometric methods for process monitoring and high-performance controller design (1992)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 38 (1992), S. 1593-1608 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A huge amount of data is collected by computer monitoring systems in the chemical process industry. Such tools as principal component analysis and partial least squares have been shown to be very effective in compressing this large volume of noisy correlated data into a subspace of much lower dimension than the original data set. Because most of what is eliminated is the collinearity of the original variables and the noise, the bulk of the information contained in the original data set is retained. The resulting low dimensional representation of the data set has been shown to be of great utility for process analysis and monitoring, as well as in selecting variables for control. These types of models can also be used directly in control system design. One way of approaching this is to use the loading matrices as compensators on the plant. Some advantages of using this approach as part of the overall control system design include automatic decoupling and efficient loop pairing, as well as natural handling of nonsquare systems and poorly conditioned systems.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690381010
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Stabilizer insights by NMR spectroscopy (1990)
    Brookfield, Conn. : Wiley-Blackwell
    Journal of Vinyl and Additive Technology 12 (1990), S. 136-141 
    Details
    ISSN: 0193-7197
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: During the past decade, advances in both hardware and software have made NMR spectroscopy an enormously powerful analytical technique. In this paper, the author presents examples of the use of NMR in support of vinyl stabilizer research; he also covers representative practical applications of proton, carbon, phosphorus, and tin NMR.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/vnl.730120304
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...