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  • 1
    Digitale Medien
    Digitale Medien
    The influence of GAP promoter variants on hirudin production, average plasmid copy number and cell growth in Saccharomyces cerevisiae (1990)
    Springer
    Current genetics 18 (1990), S. 97-103 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Heterologous gene expression ; Plasmid copy number ; GAP promoter ; Hirudin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The yeast Saccharomyces cerevisiae has been engineered to synthesize and secrete desulfato-hirudin (hirudin), a thrombin inhibitor from the leech Hirudo medicinalis. The synthetic gene coding for hirudin was expressed constitutively under the control of four size-variants of the yeast glyceraldehyde-3-phosphate dehydrogenase promoter (GAP) and cloned into a 2 μ based multicopy yeast vector. The constitutive action of the four promoter variants was confirmed by demonstrating that the expression and secretion of hirudin is growth-related. The different efficiencies of the promoter variants not only affected hirudin expression but also led to changes in several cellular parameters, such as cell growth, average plasmid copy number and plasmid stability. The observed changes show that yeast cells establish a specific equilibrium for each promoter variant. We conclude, that the adjustment of cellular parameters in response to the expression levels of a heterologous protein is regulated by two counteracting selective forces: (1) the need for complementation of the auxotrophic host marker by the plasmid-encoded selection gene which, in the case of dLEU2, requires several plasmid copies; and (2) a selective advantage of cells with a lower copy number enabling them to escape the burden of heterologous protein production.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00312596
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  • 2
    Digitale Medien
    Digitale Medien
    Rifampicin-resistance inE. coli: Comparison of microbiological and enzymatic properties (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 132-133 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01936795
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  • 3
    Digitale Medien
    Digitale Medien
    Infrared convergence of Feynman integrals for the masslessA 4-model (1976)
    Springer
    Communications in mathematical physics 46 (1976), S. 105-118 
    Details
    ISSN: 1432-0916
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik , Physik
    Notizen: Abstract For the masslessA 4-model it is proved that renormalization can be formulated such that each Feynman diagram yields an ultraviolet and infrared convergent contribution to the Green's functions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01608491
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  • 4
    Digitale Medien
    Digitale Medien
    The power counting theorem for Feynman integrals with massless propagators (1975)
    Springer
    Communications in mathematical physics 44 (1975), S. 73-86 
    Details
    ISSN: 1432-0916
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik , Physik
    Notizen: Abstract Dyson's power counting theorem is extended to the case where some of the mass parameters vanish. Weinberg's ultraviolet convergence conditions are supplemented by infrared convergence conditions which combined are sufficient for the convergence of Feynman integrals.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01609059
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  • 5
    Digitale Medien
    Digitale Medien
    Untersuchungen zum Metabolismus des Carbromal (1977)
    Springer
    Archives of toxicology 38 (1977), S. 287-294 
    Details
    ISSN: 1432-0738
    Schlagwort(e): Carbromal ; 2-Brom-2-ethylbutyrylcarbamide ; 2-Brom-2-ethyl-3-hydroxybutyramide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Erythro-2-Brom-2-ethyl-3-hydroxybutyramide has been isolated from the urines of patients with Carbromal intoxications as well as from the urine of rat, mouse and dog treated with either Carbromal or with 2-Brom-2-ethylacetamide. The identification of the excreted and synthetized metabolite was carried out by means of IR, Mass and NMR spectroscopy. Along with the erythro-2-Brom-2-ethyl-3-hydroxybutyramide one other bromine containing compound was isolated after Carbromal application only, its mass spectrum and rel. RF values having the same characteristics as the synthetized erythro-2-Brom-2-ethyl-3-hydroxybutyrylcarbamide. However, the purification of this metabolite so far has not been successful.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00352033
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  • 6
    Digitale Medien
    Digitale Medien
    Über den Nachweis von 2-Brom-2-äthyl-4-hydroxybutyramid und 2-Brom-2-äthyl-4-butyrolactam nach Carbromalgabe (1978)
    Springer
    Archives of toxicology 40 (1978), S. 119-124 
    Details
    ISSN: 1432-0738
    Schlagwort(e): Carbromal ; 2-Brom-2-ethylbutyrylcarbamide ; 2-Brom-2-ethyl-4-hydroxybutyramide ; 2-Brom-2-ethyl-4-butyrolactam
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung 2-Brom-2-äthyl-4hydroxybutyramid und 2-Brom-2-äthyl-4-butyrolactam sind aus dem Urin von Mäusen, Ratten und Hunden nach Zufuhr von Carbromal oder Bromdiäthylacetamid sowie aus dem Urin carbromalvergifteter Patienten isoliert und mittels Massen-, Kernresonanz- und IR-Spektroskopie identifiziert worden.
    Notizen: Abstract 2-Brom-2-ethyl-4-hydroxybutyramide and 2-Brom-2-ethyl-4-butyrolactam have been isolated from the urine of patients with carbromal intoxications as well as from the urine of rat, mouse and dog after the application of either carbromal or bromdiethylacetamide and identified by means of mass-, NMR- and IR spectroscopy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01891966
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  • 7
    Digitale Medien
    Digitale Medien
    N-glycosylated protein(s) are important for the infectivity of Borna disease virus (BDV) (1994)
    Springer
    Archives of virology 137 (1994), S. 405-409 
    Details
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Tunicamycin inhibited the production of infectious Borna disease virus (BDV) and glycosidase treatment eliminated the infectivity of cell-free virus. A glycoprotein of approximately 17 kDa, found in association with infectious virus, was identified by Concanavalin A binding.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01309486
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  • 8
    Digitale Medien
    Digitale Medien
    The critical velocity behavior of superfluid 4He in a micron-size aperture at two different frequencies of oscillatory flow (1993)
    Springer
    Journal of low temperature physics 91 (1993), S. 219-232 
    Details
    ISSN: 1573-7357
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Ac measurements of an effective critical velocity v scof superfluid 4He flow through a rectangular aperture 5 Μm × 0.3 Μm in a foil 0.2 Μm thick were made between 0.35 and 2.15 K. These measurements involved oscillatory flow in two different ranges of frequency, 70–118 Hz and 1818–1899 Hz. At temperatures T above 1.7 K, v sc(T) measured in the two frequency ranges coincided, decreasing with increasing T. Above 1.9 K, downward curvature toward V sc = 0 at T λoccurred in proportion to (ϱ s )/(T)0.83. At temperatures below 1.7 K, differences appeared between the critical velocity behaviors in the two frequency ranges. As temperature decreased, V sc(T) at the lower frequencies continued to rise in a nearly linear fashion, while at the higher frequencies V sc(T) tended to level off. At the same time, a pronounced increase in noise and metastability in the supercritical region was seen at the higher frequencies. Possible explanations for this divergence of behavior are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00120850
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  • 9
    Digitale Medien
    Digitale Medien
    Energy transfer and phase slip by quantum vortex motion in superfluid4He (1993)
    Springer
    Journal of low temperature physics 93 (1993), S. 1003-1018 
    Details
    ISSN: 1573-7357
    Schlagwort(e): 67.40.Hf ; 67.40.Vs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The purpose of the present article is to emphasize the usefulness of the ideas of E. R. Huggins in thinking about vortex motion and phase slip in superfluid4He, and is primarily pedagogical. Several explicit illustrations of vortex motion and phase-slip processes are considered. In addition, it is shown that Huggins's results lead to a generalization and a more complete understanding of the familiar expression E+vs · p for the energy in the rest system of an excitation in the flowing superfluid, as applied to vortex excitations. Here, E is the energy and p is the momentum of the excitation in the moving system, and vs is the superfluid velocity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00692045
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