ISSN:
1460-9568
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Muscle (body wall, buccal mass, heart) and neural tissue of the marine mollusc Aplysia californica was analysed for calcium-binding proteins using transblot45Ca overlay, Western blotting and two-dimensional polyacrylamide gel electrophoresis, and several low molecular weight calcium-binding proteins were identified. Our results show that Aplysia muscle contains an abundant protein with a Mr of ∼20 000 with strong 45Ca2+-binding ability and cross-reactivity to antibodies against the sarcoplasmic calcium-binding protein isoform II (SCP II) from Amphioxus. Immunocytochemical studies revealed that isoforms of SCP are distributed in a tissue-specific manner, SCP II-like protein is exclusively present in muscle fibres closely associated with the contractile machinery, whereas the isoform I (SCP I-like protein) is exclusively present in a subset of neurons, suggesting a function in their calcium regulation. In addition, a novel 45Ca2+-binding protein of Mr 43 000, pl 4.7, was found in muscle and in neurons. A third protein of Mr 40 000, pl 4.8, cross-reacts with anti-parvalbumin and anti-calbindin D-28K antibodies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1460-9568.1993.tb00520.x
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