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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 162 (1994), S. 414-421 
    ISSN: 1432-072X
    Keywords: Key words     Carotenoid ; Bacterioruberin ; Red pigment ; Radiotolerance ; Rubrobacter radiotolerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The highly radioresistant Rubrobacter radiotolerans, contains red pigments. Since the pigments could not be extracted by usual methods, a new method was developed in which the pigments were extracted with organic solvents after addition of 10 N KOH to the intact cells, followed by neutralization. These pigments were also extracted after treatment with achromopeptidase, but not with lysozyme. The extracted pigments separated into two main spots by TLC (48.6% and 22.6%), and were confirmed to be carotenoids by chemical tests. The two major pigments had 13 conjugated double bonds as determined from the main maximum wavelength of the light absorption spectra. Their molecular weights were determined to be 740 and 722 by mass spectrometry. The mass spectra of their TMS-derivatives revealed that they contained four and three tertiary OH groups, respectively. Confirming their identical light and IR spectra, these pigments were determined to be bacterioruberin and monoanhydrobacterioruberin, respectively, the characteristic carotenoids of halophilic bacteria. The existence of these pigments in bacteria other than halobacteria provides interesting new evidence on the distribution of these compounds.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 162 (1994), S. 414-421 
    ISSN: 1432-072X
    Keywords: Carotenoid ; Bacterioruberin ; Red pigment ; Radiotolerance ; Rubrobacter radiotolerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The highly radioresistant Rubrobacter radiotolerans, contains red pigments. Since the pigments could not be extracted by usual methods, a new method was developed in which the pigments were extracted with organic solvents after addition of 10 N KOH to the intact cells, followed by neutralization. These pigments were also extracted after treatment with achromopeptidase, but not with lysozyme. The extracted pigments separated into two main spots by TLC (48.6% and 22.6%), and were confirmed to be carotenoids by chemical tests. The two major pigments had 13 conjugated double bonds as determined from the main maximum wavelength of the light absorption spectra. Their molecular weights were determined to be 740 and 722 by mass spectrometry. The mass spectra of their TMS-derivatives revealed that they contained four and three tertiary OH groups, respectively. Confirming their identical light and IR spectra, these pigments were determined to be bacterioruberin and monoanhydrobacterioruberin, respectively, the characteristic carotenoids of halophilic bacteria. The existence of these pigments in bacteria other than halobacteria provides interesting new evidence on the distribution of these compounds.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 4 (1990), S. 381-383 
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Studies on combined supercritical-fluid chromatography (SFC) using a packed column, with fast-atom bombardment mass spectrometry (FAB-MS) are described. The interfacing was successfully carried out by simply coupling a frit interface between the liquid chromatograph (LC) and the mass spectrometer. The interface was equipped with a pneumatic splitter and post-column addition device delivering FAB matrix material to a release valve which maintained a constant back-pressure at the column outlet. Using a 1% glycerol solution as a matrix compound in the release valve, the SFC/FAB-MS combination operates as well as a normal LC/MS instrument. The performance was demonstrated with a mixture of α-, β- and γ-tocopherol and Triton X-100 (poly(ethylene glycol) p-isooctylphenyl ether).
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Letters 10 (1972), S. 51-56 
    ISSN: 0449-2986
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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